Sökning: "HRP"

Visar resultat 1 - 5 av 26 avhandlingar innehållade ordet HRP.

  1. 1. Bifunctional ADAPTs: Opportunity for serological Half-life extension and Targeted therapy

    Författare :Andreas Wisniewski; Sophia Hober; Masood Kamali-Moghaddam; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Autoimmune diseases; Cancer; Half-life extension; HRP; Phage Display; Protein Engineering; Targeted therapy; Biotechnology; Bioteknologi;

    Sammanfattning : Small engineered scaffold proteins (ESPs) gain more and more popularity as biological drugs, due to their specificity and applicability in diagnostics and therapy. Thanks to their high stability, low immunogenicity and low production cost, they present themselves as a promising alternative to the market-leading antibodies. LÄS MER

  2. 2. RECOMBINANT PEROXIDASES AND XYLANASES: I. Cloning and production of a peroxidase from horseradish II. Characterisation of functional domains of thermostable xylanases from Rhodothermus marinus

    Författare :Eva Bartonek Roxå; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amorphous cellulose; xylan; CBD; carbohydrate binding domains; insect cells; Baculovirus; catalytically active; recombinant horseradish peroxidase; HRP-n; PASC; beta-glucan; Biotechnology; Bioteknik;

    Sammanfattning : Part I: Plant peroxidases are useful in many areas of application e.g. as tracer enzymes for clinical applications and in biosensors as well as for industrial purposes like in waste water treatment and bleaching processes. A gene encoding a new, neutral horseradish peroxidase isoenzyme, HRP-n, was isolated. LÄS MER

  3. 3. Amperometric Carbon Paste Biosensors Based on Horseradish Peroxidase and Oxidases

    Författare :Vijayakumar Rajendran; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; on-line monitoring; l-lactate; glucose; ethanol; Biosensor; Carbon paste; Analytisk kemi;

    Sammanfattning : Amperometric carbon paste peroxide sensors based on horseradish peroxidase (HRP) were developed. In the first approach, HRP was chemically modified with polyethylene glycol to improve the stability of the enzyme in the organic paste matrix and compared with native HRP electrode. LÄS MER

  4. 4. Crystallography in Four Dimensions : Methods and Applications

    Författare :Gunilla Carlsson; Janos Hajdu; Dieter Oesterhelt; Vilmos Fülöp; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biophysics; horseradish peroxidase; redox reactions; X-ray crystallography; haem catalysis; molecular movies; radiation damage; Molekylär biofysik; Molecular biophysics; Molekylär biofysik;

    Sammanfattning : The four-electron reduction of dioxygen to water is the most exothermic non-photochemical reaction available to biology. A detailed molecular description of this reaction is needed to understand oxygen-based redox processes. Horseradish peroxidase (HRP) is a haem-containing redox enzyme capable of catalysing the reduction of dioxygen to water. LÄS MER

  5. 5. Neuronal tracers for fine caliber spinal primary afferents and their response to peripheral nerve injury

    Författare :H Fredrik Wang; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Rat; Primary afferents; Dorsal root ganglion; Peripheral nerve injury; Spinal cord; Isolectin GSA I-B4; Soybean agglutinin; Cholera toxin B subunit; Horseradish peroxidase; Immunohistochemistry; Neuronal tracing;

    Sammanfattning : The isolectin B4 from Griffonia simplicifolia I (B4) and soybean agglutinin from the plant Glycine max (SBA), have been shown to bind to rat small-diameter dorsal root ganglion (DRG) neurons, and to fibers and presumed terminals in laminae I-II of the spinal cord dorsal horn. In the present thesis, these lectins and/or their horseradish peroxidase (HRP) conjugates, have been investigated in the rat with regard to their usefulness as selective neuronal markers of and axonally transported tracers for somatic and/or visceral spinal primary afferents with fine caliber, including unmyelinated, axons. LÄS MER