Sökning: "30S subunit"

Visar resultat 6 - 10 av 15 avhandlingar innehållade orden 30S subunit.

1. 6. Study of the Link between Translation Termination and Ribosome Biogenesis

   Författare :Magdalena Kaczanowska; Monica Rydén Aulin; Catherine L. Squires; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microbiology; Mikrobiologi;

   Sammanfattning : Release factor 1 (RF1) is a ribosome-binding protein required for termination of translation in Escherichia coli. To study the effect of slow termination on protein synthesis, two isolated suppressors to a temperature sensitive (Ts) mutant allele of RF1 were studied. LÄS MER

3. 7. Ribosomes and subunits from Escherichia coli studied by asymmetrical flow field-flow fractionation

   Författare :Mikael Nilsson; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolic engineering; VHb; Vitreoscilla hemoglobin; E. coli; Escherichia coli; separation; subunits; ribosomes; Asymmetrical flow field-flow fractionation; flow FFF; optimization; biotechnological production; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis asymmetrical flow field-flow fractionation (FFF) separations of ribosomes and subunits have been studied. Ribosomal material was prepared from Escherichia coli (E. coli) cells and analyzed by the size separation technique. LÄS MER

4. 8. Finding the unknowns in trans-translation

   Författare :Natalia Ivanova; Måns Ehrenberg; Nenad Ban; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; trans-translation; tmRNA; SmpB; kinetics; footprinting; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Sammanfattning : Ribosomes stalled on problematic mRNAs can be rescued by a mechanism called trans-translation. This mechanism employs a dual transfer-messenger RNA molecule (tmRNA) together with a helper protein (SmpB). In this work we have used an in vitro translation system with pure components to further clarify the roles of tmRNA and SmpB in trans-translation. LÄS MER

5. 9. From Structure to Function with Binding Free Energy Calculations for Codon Reading, Riboswitches and Lectins

   Författare :Johan Sund; Åqvist Johan; Javier Luque; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; binding free energy; ribosome; codon reading; release factor; mitochondrial translation; purine riboswitch; lectin; molecular dynamics; free energy perturbation; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

   Sammanfattning : Molecular association is part of many important processes in living cells. Computational methods for calculating binding free energies allows for a quantitative examination of biomolecular structures and hypotheses drawn from biochemical experiments. LÄS MER

7. 10. Experimental Evolution : and Fitness Effects of Mutations

   Författare :Anna Knöppel; Dan I Andersson; Stanley Maloy; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Experimental evolution; Fitness effects; mutations; S20; fis; ribosome; horizontal gene transfer; adaptation; synonymous mutations; Mikrobiologi; Microbiology;

   Sammanfattning : Bacteria have small, streamlined genomes and evolve rapidly. Their large population sizes allow selection to be the main driver of evolution. With advances in sequencing technologies and precise methods for genetic engineering, many bacteria are excellent models for studying elementary questions in evolutionary biology. LÄS MER