Sökning: "single molecule"

Visar resultat 6 - 10 av 430 avhandlingar innehållade orden single molecule.

  1. 6. Probing Biomolecular Recognition at the Single Molecule Level

    Författare :Anders Gunnarsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cell membrane; TIRFM; liposome; time-of-flight secondary ion mass spectrometry; membrane receptor; single molecule; TOF-SIMS; molecular recognition; DNA; barcode; total internal reflection; multiplexing;

    Sammanfattning : Specific recognition between biomolecular partners contributes to a multitude of biochemical signaling within and between cells. The aim of this work has been to investigate the possibility to probe biomolecular recognition reactions at the smallest possible scale, the single molecule level. LÄS MER

  3. 7. Overstretching short DNA – Single-molecule force spectroscopy studies

    Författare :Niklas Bosaeus; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA stretching; mechanical properties of DNA; single molecule; optical tweezers; DNA structure;

    Sammanfattning : Structural deformation of DNA has a central role in many biological processes. It occurs, for example, during replication, transcription, and regulation of the activity of the genome. To understand these fundamental processes it is necessary to have a detailed knowledge of the mechanical properties of DNA. LÄS MER

  4. 8. Detection and Sequencing of Amplified Single Molecules

    Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

  5. 9. Single-­molecule spectroscopy of π-­conjugated polymers

    Författare :Oleg Mirzov; Kemisk fysik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence lifetime; low temperature; spectral diffusion; blinking; MEH-PPV; Single-molecule spectroscopy; π-conjugated polymers; 2D polarization; Monte Carlo; energy transfer;

    Sammanfattning : When applied to condensed-matter samples of large organic molecules, optical spectroscopy faces the problem of inhomogeneous broadening caused by the diversity of environments and conformations that sample molecules are subjected to. This severely limits the information that can be extracted from optical spectra. LÄS MER

  7. 10. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

    Författare :Olle Ericsson; Ulf Landegren; Mats Nilsson; Edwin Southern; Uppsala universitet; []
    Nyckelord :Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

    Sammanfattning : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. LÄS MER