Sökning: "TIRFM"

Hittade 4 avhandlingar innehållade ordet TIRFM.

  1. 1. Probing Biomolecular Recognition at the Single Molecule Level

    Författare :Anders Gunnarsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cell membrane; TIRFM; liposome; time-of-flight secondary ion mass spectrometry; membrane receptor; single molecule; TOF-SIMS; molecular recognition; DNA; barcode; total internal reflection; multiplexing;

    Sammanfattning : Specific recognition between biomolecular partners contributes to a multitude of biochemical signaling within and between cells. The aim of this work has been to investigate the possibility to probe biomolecular recognition reactions at the smallest possible scale, the single molecule level. LÄS MER

  3. 2. Virus-cell membrane interactions - Binding studies of Herpes Simplex Virus using surface-sensitive techniques

    Författare :Nadia Peerboom; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; single particle tracking; binding kinetics; glycosaminoglycans; herpes simplex virus;

    Sammanfattning : Viruses are parasites capable of infecting all forms of life. They lack the ability to replicate by themselves and therefore hijack the replication machinery of cells to produce new viral copies, called virions. LÄS MER

  4. 3. Studies on interactions of norovirus capsid protein with fucosylated glycans and galactosylceramide as soluble and membrane bound ligands

    Författare :Waqas Nasir; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Norovirus-glycan interactions;

    Sammanfattning : Noroviruses (NVs) are among the most common viral pathogens which target the gastrointestinal (GI) tract and cause severe diarrhea, vomiting and episodes of abdominal cramps with fever. Millions of people around the world get infected with NVs annually, of which 200,000 cases are estimated to be fatal. LÄS MER

  5. 4. Tethered Lipid Vesicles as Nanoscale Sensor Elements

    Författare :Seyed Tabaei; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaglyceroporin; lipid vesicles; total internal reflection fluorescence TIRF microscopy; cell membrane; aquaporin; pore formation; surface plasmon resonance SPR ; phospholipase A2; antiviral peptide; lipid bilayer; biosensing; microfluidics;

    Sammanfattning : The primary aim of this thesis work has been to explore and advance the potential of surface tethered lipid vesicles as a platform for studying in particular the following three biomembrane-related processes: trans-membrane transport, membrane-peptide interactions and the activity of membrane active enzymes. First, a method based on surface plasmon resonance (SPR) spectroscopy was developed for studying passive diffusion processes as well as the membrane-protein mediated transport of small non electrolyte molecules across lipid bilayers. LÄS MER