Sökning: "single molecule"

Visar resultat 21 - 25 av 430 avhandlingar innehållade orden single molecule.

  1. 21. High Content Analysis of Proteins and Protein Interactions by Proximity Ligation

    Författare :Karl-Johan Leuchowius; Ola Söderberg; Ulf Landegren; Paul Goldsmith; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation; flow cytometry; high content screening; rolling circle amplification; in situ PLA; single-cell; single-molecule; protein interactions; drug screening; post-translational modifications; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. LÄS MER

  3. 22. Making Visible the Proximity Between Proteins

    Författare :Carl-Magnus Clausson; Ola Söderberg; Jens Coorssen; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proximity ligation assay; In situ PLA; rolling circle amplification; protein interaction; protein-protein interaction; in situ; single cell; single molecule; protein complex; antibody; cancer; tissue section; microscopy; image analysis; system biology; multiplex; dynamic range; methods development; systems biology; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. LÄS MER

  4. 23. From single-molecule sensing to extracellular vesicles in glioma cells under stress

    Författare :Virginia Claudio; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteome; single molecule sensing; nanoplasmonics; surface plasmon resonance; exosomes; glioblastoma; extracellular vesicles; cellular stress; lipid composition;

    Sammanfattning : This thesis describes the work I conducted in two different areas, namely biomolecular sensing and quantitative cell biology. The work in the former area was focused on the optimization of plasmonic metamaterials for sensing applications, and in the latter on the characterization of extracellular vesicles released by glioblastoma cells under stress conditions. LÄS MER

  5. 24. Visualizing Interacting Biomolecules In Situ

    Författare :Irene Weibrecht; Ola Söderberg; Ulf Landegren; Banafshe Larijani; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ PLA; padlock probe; rolling circle amplification; flow cytometry; in situ; single cell; single molecule; protein interaction; protein-DNA interaction; posttranslational modification; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. LÄS MER

  7. 25. Surface Raman spectroscopy of Ag-nanoparticles : single molecules and monolayers

    Författare :Erik J. Bjerneld; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface-enhanced Raman scattering SERS ; single-molecule; silver; nanoparticle;

    Sammanfattning : .... LÄS MER