Sökning: "in vitro evolution"

Visar resultat 1 - 5 av 91 avhandlingar innehållade orden in vitro evolution.

  1. 1. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins

    Författare :Mikael Nilsson; Mikael Widersten; Dan Tawfik; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; In vitro evolution; Phage display; mRNA display; Cro; DNA recognition; repressor; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER

  3. 2. Molecular Evolution of Neuropeptide Y Receptors in Vertebrates

    Författare :Erik Salaneck; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurosciences; Neuropeptide Y; Receptor; Evolution; Neurovetenskap; Neurology; Neurologi; Neurology; neurologi;

    Sammanfattning : The three evolutionarily related peptides neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP) are ligands to at least five G-protein coupled receptors in mammals, which are denoted by numbers. NPY has many physiological effects including stimulation of appetite and regulation of circadian rhythm and blood pressure. LÄS MER

  4. 3. In vivo and in vitro evolution of molecular mechanisms - Importance in B cell development and phage display

    Författare :Nina Nilsson; Institutionen för Immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; B cell development and phage display; transplantation; serology; Immunology; Immunologi; serologi; Biology; Biologi;

    Sammanfattning : Bone marrow resident hematopoietic stem cells differentiate into all different types of lymphoid populations, there among mature naive B cells expressing IgM and IgD. During the maturation, the B cells are subjected to several steps of selection to ensure proper development of the antigen receptor. LÄS MER

  5. 4. Evolution of olfaction in Lepidoptera and Trichoptera : Gene families and antennal morphology

    Författare :Jothi Kumar Yuvaraj; Funktionell zoologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; evolution; pheromone receptors; olfaction; chemosensory; sensillum; HEK 293 cells;

    Sammanfattning : In moths, females produce sex pheromone compounds to attract males over a long distance for mating. The antennae of moths and many other insects have specialized odorant receptors (ORs), called pheromone receptors (PRs), to sense the pheromone compounds and they group in a monophyletic clade (PR clade). LÄS MER

  7. 5. Biochemical Studies on a Plant Epoxide Hydrolase : Discovery of a Proton Entry and Exit Pathway and the Use of In vitro Evolution to Shift Enantioselectivity

    Författare :Ann Gurell; Mikael Widersten; Bengt Mannervik; Dick Janssen; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; epoxide hydrolase; enantioselectivity; in vitro evolution; proton wire; epoxides; selectivity; CASTing; structure-function; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : The work leading to this thesis has provided additional information and novel knowledge concerning structure-function relationship in the potato epoxide hydrolase. Epoxide hydrolases are enzymes catalyzing the hydrolysis of epoxides to yield the corresponding vicinal diols. LÄS MER