Sökning: "periplasm"
Visar resultat 1 - 5 av 24 avhandlingar innehållade ordet periplasm.
1. Production and folding of proteins in the periplasm of Escherichia coli
Sammanfattning : The Gram-negative bacterium E. coli is the most widely used host for the production of recombinant proteins. Disulfide bond containing recombinant proteins are usually produced in the periplasm of E. coli since in this compartment of the cell - in contrast to the cytoplasm - disulfide bond formation is promoted. LÄS MER
2. Nitric Oxide Reductase from Paracoccus denitrificans : A Proton Transfer Pathway from the “Wrong” Side
Sammanfattning : Denitrification is an anaerobic process performed by several soil bacteria as an alternative to aerobic respiration. A key-step in denitrification (the N-N-bond is made) is catalyzed by nitric oxide reductase (NOR); 2NO + 2e- + 2H+ → N2O + H2O. LÄS MER
3. Membrane and secretory protein production in Escherichia coli
Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER
4. The Structural Modularity and Inherent Dynamics of the DegP Protease together with its Intertwined Role in the Bacterial Periplasm
Sammanfattning : The protein quality control machinery is a delicate and integrated network of molecular tools working together to fold or remove unwanted proteins from the cell. A distinct set of “gatekeepers” are involved in this process including molecular chaperones, proteases, oxidoreductases, transferases, and many others in both eukaryotic and prokaryotic organisms. LÄS MER
5. Studies of pore-forming bacterial protein toxins in Escherichia coli
Sammanfattning : Escherichia coli, a Gram-negative bacterium, which can be classified into three groups: the commensal, intestinal pathogenic (IPEC) and extra-intestinal pathogenic (ExPEC) E. coli. The cytolysin A (ClyA) protein, a 34-kDa pore-forming toxin, encoded by a gene found in both non-pathogenic and pathogenic E. LÄS MER