Sökning: "protein-protein proximity"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden protein-protein proximity.

1. 1. Making Visible the Proximity Between Proteins

   Författare :Carl-Magnus Clausson; Ola Söderberg; Jens Coorssen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proximity ligation assay; In situ PLA; rolling circle amplification; protein interaction; protein-protein interaction; in situ; single cell; single molecule; protein complex; antibody; cancer; tissue section; microscopy; image analysis; system biology; multiplex; dynamic range; methods development; systems biology; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. LÄS MER

3. 2. Analysis of PDGF receptor internalization and signaling using proximity ligation assays

   Författare :Marie Rubin Sander; Ola Söderberg; Cristina Al-Khalili Szigyarto; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; PDGFRβ; dynamin; proteolytic cleavage; signaling; internalization; protein-protein interaction; short linear motif; viral hijack; in situ PLA; Molboolean;

   Sammanfattning : Cell signaling is mediated by signaling proteins that relay the signal in an intricate network of interactions before the signal is translated into a biological response. Short linear motifs (SLiMs) in intrinsically disordered regions of proteins serve as docking sites for protein interaction in all aspects of cell regulation including signal transduction. LÄS MER

4. 3. Identification of stem cell factors - Novel protein-protein interactions and their functions

   Författare :Helena Johansson; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; embryonic stem cells; Tpt1; Npm1; Ncl; Oct4; Sox2; Nanog; SAF-A; cell proliferation; transcriptional regulation;

   Sammanfattning : Embryonic stem (ES) cells provide an unlimited source of cells potentially useful for regenerative medicine, however, prior to clinical implementation, additional basic research is needed. This thesis is focused upon different molecular aspects regarding ES cells, primarily by finding novel stem cell protein-protein interactions and their functions. LÄS MER

5. 4. Organization and regulation of mitochondrial gene expression

   Författare :Roger Salvatori; Martin Ott; Benoit Kornmann; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mitochondria; mitochondrial gene expression; post-transcriptional regulation; mitochondrial ribosome; protein-protein proximity; BioID; feedback loop; Biochemistry; biokemi;

   Sammanfattning : Mitochondria possess their own genome, remnant of the ancestral eubacterial endosymbiont DNA. This mitochondrial genome encodes mostly few key subunits of the respiratory chain. In order to synthesize these few proteins, mitochondria contain a complete gene expression machinery. LÄS MER

7. 5. Development of DNA-based methods for analysis of protein interactions

   Författare :Axel Klaesson; Ola Söderberg; Mats Nilsson; Cristina Al-Khalili Szigyarto; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; In situ proximity ligation assay PLA ; rolling circle amplification RCA ; Hybridization chain reaction HCR ; proxHCR; Oligonucleotide design; Protein-protein interactions; Post-translational modifications.; Farmaceutisk vetenskap; Pharmaceutical Science; Molekylär cellbiologi; Molecular Cellbiology;

   Sammanfattning : In situ proximity ligation assay (PLA) is a method for detection of protein interactions, post-translational modifications (PTMs) and individual proteins that allows information about their localization in a cell or tissue to be extracted. The method is based on oligonucleotide-conjugated antibodies (proximity probes) that upon binding of two epitopes in close proximity give rise to an amplifiable DNA circle. LÄS MER