Sökning: "proxHCR"

Hittade 3 avhandlingar innehållade ordet proxHCR.

  1. 1. Development of DNA-based methods for analysis of protein interactions

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Axel Klaesson; Uppsala universitet.; [2018]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; In situ proximity ligation assay PLA ; rolling circle amplification RCA ; Hybridization chain reaction HCR ; proxHCR; Oligonucleotide design; Protein-protein interactions; Post-translational modifications.; Farmaceutisk vetenskap; Pharmaceutical Science; Molekylär cellbiologi; Molecular Cellbiology;

    Sammanfattning : In situ proximity ligation assay (PLA) is a method for detection of protein interactions, post-translational modifications (PTMs) and individual proteins that allows information about their localization in a cell or tissue to be extracted. The method is based on oligonucleotide-conjugated antibodies (proximity probes) that upon binding of two epitopes in close proximity give rise to an amplifiable DNA circle. LÄS MER

  2. 2. Improvements and Applications of in situ Proximity Ligation Assays

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Karin Grannas; Uppsala universitet.; [2015]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molekylär medicin; Molecular Medicine;

    Sammanfattning : The cells building up the human body is in constant communication with each other. This communication is done through large complex networks of signaling pathways for inter- and intracellular signal transduction. The signaling activity regulates many important processes, for example cell death, proliferation and differentiation. LÄS MER

  3. 3. Applications of in situ proximity ligation assays for cancer research and diagnostics

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Liza Löf; Uppsala universitet.; [2016]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cancer; Diagnostics; Research; Prognostics; Method development; Flow cytometry; Drug delivery; Molekylär medicin; Molecular Medicine;

    Sammanfattning : In the field of cancer research and diagnostics it is crucial to have reliable methods for detecting molecules involved in the disease. New and better methods for diagnostics, prognostics and drug delivery therefore remain a permanent aim. LÄS MER