Sökning: "protein stabilization"

Visar resultat 1 - 5 av 105 avhandlingar innehållade orden protein stabilization.

1. 1. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

   Författare :Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. LÄS MER

3. 2. Protein stabilization. Some methods and mechanisms

   Författare :Maria Andersson; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; sorbitol; protein stability; preferential exclusion; polyol; poly-NIPAAm; polymer; poly ethyleneimine ; polyelectrolyte; oxidation; NMR spectroscopy; lysozyme; lactate dehydrogenase; fluorescence; dynamic light scattering; complex; additive; circular dichroism; stabilization; trypsin; thermo-reversible polymer.; Biotechnology; Bioteknik;

   Sammanfattning : The effect of different additives on protein stability was studied, and the basis for stabilization was investigated at molecular level. The addition of sorbitol enhanced thermal stability of hen egg white lysozyme in aqueous solution. LÄS MER

4. 3. Protein engineering to explore and improve affinity ligands

   Författare :Martin Linhult; KTH; []
   Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

   Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

5. 4. Unravelling protein stability and aggregation

   Författare :Tanja Weiffert; NanoLund: Centre for Nanoscience; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein aggregation; amyloid beta peptide; protein stabilization; split GFP; monellin; secretagogin; ; aggregation kinetics; toxicity; aggregation mechanism; coaggregation;

   Sammanfattning : Proteins are complex macromolecules that are fundamental to all living species. The stability of proteins are governed by non-covalent forces within the protein and between the protein and its surrounding environment. LÄS MER

7. 5. The Role of Sugars for Protein Stabilization

   Författare :Christoffer Olsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein; water; QENS; biomolecule; EPSR; amorphous; trehalose; neutron scattering; cryopreservation; DSC; neutron diffraction; dielectric spectroscopy;

   Sammanfattning : The understanding of biomolecular interactions with water and co-solutes can lead to greater knowledge regarding the mechanisms behind biomolecular stabilization. This is highly important for developing technologies aimed to preserve biological materials. LÄS MER