Sökning: "protein specificity"

Visar resultat 6 - 10 av 567 avhandlingar innehållade orden protein specificity.

  1. 6. Studies on the Differential Specificity of Protein Kinases and Its Applications

    Författare :Mart Loog; Bengt Jergil; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Protein kinases; protein kinase inhibitors; substrate specificity; peptide libraries; affinity chromatography; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Protein kinases are enzymes that catalyse the phosphoryl transfer from the g-phosphate of ATP to acceptor amino acids in proteins. The specificity of selected model protein kinases was studied at three different levels using a) novel bi-substrate-analogue inhibitors, b) synthetic peptide substrates and c) mutated protein substrate analogues. LÄS MER

  3. 7. DNA-Assisted Immunoassays for High-Performance Protein Analyses

    Författare :Junhong Yan; Masood Kamali-Moghaddam; Ulf Landegren; Christer Wingren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; magnetic beads; conjugation; phosphorylation; affinity binder; protein complexes; recombinant binder; sensitivity; specificity; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Proteins play important roles in most cellular functions, such as, replication, transcription regulation, signal transduction, for catalyzing chemical reaction, etc. Technologies developed to identify proteins rely either on observing their own properties such as charge, size, mass to charge ratio or sequence composition; or on using affinity reagents that recognize specific protein targets. LÄS MER

  4. 8. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

    Författare :Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. LÄS MER

  5. 9. Mapping and annotating the mammalian body-wide protein-coding gene expression

    Författare :Max Karlsson; Mathias Uhlén; Linn Fagerberg; Julio Saez-Rodriguez; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein; protein-coding; genes; annotation; atlas; scRNA-Seq; RNA-Seq; transcriptomics; proteomics; Biotechnology; Bioteknologi;

    Sammanfattning : A central aim of fundamental research is to create conditions necessary for fueling further research and innovation. Our understanding of basic biology is central for future developments of tools for diagnosing, monitoring, and treating disease. LÄS MER

  7. 10. Anticoagulant protein C--structural and functional studies

    Författare :Lei Shen; Malmö Klinisk kemi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; enzymology; Proteins; Klinisk kemi; Clinical chemistry; serpins; membrane binding; factor V; protein C; protein S; Proteiner; enzymologi;

    Sammanfattning : Anticoagulant protein C is a vitamin K-dependent factor circulating in plasma as a zymogen of a serine protease. It is converted to its active form (APC) by the thrombin-thrombomodulin complex on the surface of endothelial cells. APC inhibits the generation of thrombin by selective proteolytic inactivation procoagulant factors Va and VIIIa. LÄS MER