Sökning: "peptide libraries"

Visar resultat 1 - 5 av 29 avhandlingar innehållade orden peptide libraries.

  1. 1. Combinatorial Methodology. Screening for affinity specific ligands to biological and artificial receptors

    Författare :Margareta Krook; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; peptide library; combinatorial; screening; phage display; affinity; DNA-interaction; chymotrypsin; peptide inhibitor; antibody; IgG; Fc; molecular imprinting; steroid; receptor; Biochemistry; Metabolism; Biokemi; Biotechnology; Bioteknik;

    Sammanfattning : Combinatorial screening methodology has been employed to select new affinity specific ligands to bilogical and artificial receptors. The screening methodology, in particular screening of combinatorial phage display peptide libraries against target molecules differing in function and shape, is focused upon. LÄS MER

  3. 2. Studies on the Differential Specificity of Protein Kinases and Its Applications

    Författare :Mart Loog; Bengt Jergil; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Protein kinases; protein kinase inhibitors; substrate specificity; peptide libraries; affinity chromatography; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Protein kinases are enzymes that catalyse the phosphoryl transfer from the g-phosphate of ATP to acceptor amino acids in proteins. The specificity of selected model protein kinases was studied at three different levels using a) novel bi-substrate-analogue inhibitors, b) synthetic peptide substrates and c) mutated protein substrate analogues. LÄS MER

  4. 3. Development of a novel methodology for the synthesis of oligonucleo-peptide conjugates

    Författare :Simone Zaramella; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Antisense; Oligonucleotide-peptide conjugates; Solid-phase synthesis; Histidine; Protecting groups.;

    Sammanfattning : Peptide-oligonucleotide conjugates constitute a new class of antisense molecules that exhibit improved biological activity over standard oligonucleotide analogues. Currently, these oligomeric bioconjugates are prepared by either solution- or solid-phase techniques. LÄS MER

  5. 4. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  7. 5. Molecular basis and pharmacological implications of Alzheimer amyloid ß-peptide fibril formation

    Författare :Lars Tjernberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Alzheimer s disease; amyloid; fibrils; inhibitor; protease resistance; electron microscopy; fluorescence correlation spectroscopy.;

    Sammanfattning : Alzheimer's disease is a progressive neurodegenerative disease, mostly affecting elderly. The invariable deposition of protease-resistant fibrils of Alzheimer amyloid ß-peptide (Aß) in the parenchyma and blood vessels of the brain is a central event. LÄS MER