Sökning: "protein immobilization."
Visar resultat 1 - 5 av 71 avhandlingar innehållade orden protein immobilization..
1. Immobilization and Interaction of Biomolecules at the Solid-Liquid Interface : Applications to Solid-Phase Analytical Techniques
Sammanfattning : The development of solid-phase-analytical techniques based on surface-concentration-measuring devices requires a stable and reproducible immobilization of biomolecules on well-characterized solid surfaces. This study reports on the immobilization of Immunoglobulin G, Protein A and Fibronectin on chemically modified silica surfaces. LÄS MER
2. Biosensor surface chemistry for oriented protein immobilization and biochip patterning
Sammanfattning : This licentiate thesis is focused on two methods for protein immobilization to biosensor surfaces for future applications in protein microarray formats. The common denominator is a surface chemistry based on a gold substrate with a self-assembled monolayer (SAM) of functionalized alkanethiolates. LÄS MER
3. Macromolecules at Interfaces
Sammanfattning : In this thesis, the structure and stability of globular proteins adsorbed onto nanometer-sized hydrophilic silica particles were investigated using differential scanning calorimetry (DSC), hydrogen/deuterium exchange (HDX), and mass spectrometry (MS). The adsorption process itself was characterized with fluorescence and absorption spectroscopy and surface plasmon resonance (SPR). LÄS MER
4. Immobilization of feruloyl esterases in mesoporous silica
Sammanfattning : Feruloyl esterases are a class of enzymes that can be used as biocatalysts for the modification of bioactive hydroxycinnamic acids. In industry, enzyme immobilization is often necessary to stabilize the enzymes and to allow their reuse. LÄS MER
5. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics
Sammanfattning : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. LÄS MER