Sökning: "Jerker Widengren"

Visar resultat 1 - 5 av 19 avhandlingar innehållade orden Jerker Widengren.

  1. 1. Fluorescence correlation spectroscopy, photophysical aspects and applications

    Författare :Jerker Widengren; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Fluorescence correlation spectroscopy (FCS) is a technique where dynamic processes on the molecular level are studied by the use of fluorescence. The molecules are excited within a focused stationary laser beam and the resulting fluorescence fluctuations are analyzed in the form of an autocorrelation function. LÄS MER

  3. 2. Super resolution fluorescence imaging : analyses, simulations and applications

    Författare :Jan Bergstrand; Jerker Widengren; Eilon Sherman; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physics; Fysik;

    Sammanfattning : Fluorescence methods offer extraordinary sensitivity and specificity, and are extensively used in the life sciences. In recent years, super resolution fluorescence imaging techniques have developed strongly, uniquely combining ~10 nm sub diffraction resolution and specific labeling with high efficiency. LÄS MER

  4. 3. Photo-induced dark states influorescence spectroscopy – investigations & applications

    Författare :Andriy Chmyrov; Jerker Widengren; Johan Hofkens; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence correlation spectroscopy; triplet state; isomerisation; photobleaching; quenching; diffusion; total internal reflection; interface; Spectroscopy; Spektroskopi;

    Sammanfattning : This thesis focuses on investigations of transient dark states of fluorescentmolecules using spectroscopic techniques. The main purpose is to show andconvince the reader that transient dark states are not always a nuisance, butalso represent an additional source of information. LÄS MER

  5. 4. Fluorescence fluctuation studies of biomolecular interactions in solutions, biomembranes and live cells

    Författare :Volodymyr Chmyrov; Jerker Widengren; Antoine Delon; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FCS; FCCS; Isomerization; TRAST; NMR; FRET; biomombrane; fluidity; Biological Physics; Biologisk fysik;

    Sammanfattning : Fluorescence spectroscopy and imaging have a very broad spectrum of applicationswithin the life sciences, in particular for detection and characterization ofbiomolecular dynamics and interactions in different environments. This thesis comprisesprojects that strive to further expand the information content extracted fromthe detected fluorescence, leading to sensitive readout parameters for studies ofbiomolecular dynamics and interactions. LÄS MER

  7. 5. Concepts and biomedical applications of excitation-modulated transient state monitoring of fluorescence emitters

    Författare :Baris Demirbay; Jerker Widengren; Yves Mely; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik;

    Sammanfattning : Fluorescence methods have developed very strongly in the last decade, allowing single- molecule detection sensitivity, high specificity, high time- and spatial resolution, as well as high readout speeds. Together, this makes fluorescencea very central readout modality for biomolecular and cellular studies. LÄS MER