Sökning: "protein extraction"

Visar resultat 1 - 5 av 118 avhandlingar innehållade orden protein extraction.

  1. 1. Nuclear envelope protein interaction studies

    Författare :Mohammed Hakim Jaffer Ali; Einar Hallberg; Anki Östlund-Farrants; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Samp1; Nuclear envelope; protein interactions; chemical crosslinking; proteomics; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Sammanfattning : The nuclear envelope (NE) separating the nucleoplasm from cytoplasm consists of two concentric lipid membranes, the outer (ONM) and inner (INM) nuclear membranes, the nuclear pore complexes (NPCs) and an underlying nuclear lamina network. The INM contains more than 100 unique transmembrane proteins of which only a few have been characterized. LÄS MER

  3. 2. Food ingredients from cultivated seaweeds-Improving storage stability and protein recovery

    Författare :Hanna Harrysson; Chalmers University of Technology; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; alterative cultivation; pH-shift; proteins; lipid oxidation; protein extraction; unsaturated fatty acids; ascorbic acid; long-term storage; sequential recovery; Seaweed; macroalgae;

    Sammanfattning : There is a global demand for new vegetarian protein sources, and seaweed have for multiple reasons been identified as a promising candidate. The overall aim of this thesis was to evaluate the potential of three different Swedish seaweed species as food protein sources, but also as sources of unsaturated fatty acids, vitamins and minerals. LÄS MER

  4. 3. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Författare :Sheryl Fernandes; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER

  5. 4. Validation of antibodies for protein profiling : A study using immunohistochemistry on tissue microarrays

    Författare :Linda Paavilainen; Fredrik Pontén; Ola Nilsson; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; antibody validation; monospecific antibodies; protein profiling; immunohistochemistry; tissue microarrays; western blot; fixatives; Pathology; Patologi; Pathology; patologi;

    Sammanfattning : The field of proteomics has rapidly expanded due to the completion of the human genome sequence. This thesis validates affinity-purified monospecific antibodies of polyclonal origin, for protein profiling in a broad spectrum of normal tissues and cells. LÄS MER

  7. 5. Polysaccharides from red and green seaweed : Extraction, characterisation and applications

    Författare :Niklas Wahlström; Ulrica Edlund; Fredrik Gröndahl; Federica Chiellini; KTH; []
    Nyckelord :biorefinery; seaweed; macroalgae; Ulva; Porphyra; ulvan; porphyran; cellulose; gelatin; hydrogel; heavy metal; methylene blue; Fiber- och polymervetenskap; Fibre and Polymer Science;

    Sammanfattning : Cultivation of seaweed has been conducted for centuries in Asia, but is largely undeveloped in Sweden even though there is potential for this industry. Seaweed offers benefits compared with land-based biomass cultivation. LÄS MER