Sökning: "protein extraction"

Visar resultat 11 - 15 av 142 avhandlingar innehållade orden protein extraction.

1. 11. Method and technique development in peptide and protein mass Spectrometry

   Författare :Kristina Håkansson; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; electrospray ionization; peptide sequencing; Fourier transform ion cyclotron resonance; hydrogen deuterium exchange; protein structure; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Mechanistic studies and applicability of several methods and techniques for studying peptides and proteins with mass spectrometry are presented. A model for the protein molecular ion formation in explosive matrix-assisted plasma desorption mass spectrometry is proposed, involving a contribution from the chemical energy release during conversion of the explosive, an enhanced protonation through collisions with products from the explosive decay, and electron scavenging by other products. LÄS MER

3. 12. Miniaturized, Membrane-Based and Environmentally Green Sample Preparation Systems: Applications for Biological and Environmental Aqueous Samples

   Författare :Thaer Barri; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytisk kemi; Analytical chemistry; Free concentration; Exhustive extraction; Microporous membrane liquid-liqid extraction; Miniaturization; Supported liquid membrane extraction; Environmental chemistry; Miljökemi;

   Sammanfattning : Contemporary advancements in miniaturization of analytical systems have advantageously fostered development, automation, and hyphenation of a variety of sample preparation techniques. Among several downscaled extraction designs, porous synthetic polymeric membranes (such as flat sheet (FS) or hollow fiber (HF)) have been utilized for membrane-based extraction, separating two aqueous phases (as in supported liquid membrane (SLM) extraction or one aqueous phase and another organic phase (as in microporous membrane liquid-liquid extraction (MMLLE)). LÄS MER

4. 13. High-throughput screening of solid-phase extraction materials using mass spectrometry

   Författare :Kishore Jagadeesan; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :mass spectromery; protein separation; sample preparation; Molecularly imprinted polymer; Data analysis; Data visualization; Miniaturisation; Proteomics;

   Sammanfattning : In biomarker analysis, sample preparation plays a crucial step in order to extract, isolate and concentrate the analytes of interest. Among the various sample preparation techniques, solid phase extraction (SPE) is one of the most common and popular, due to the high enrichment factor, good recovery, low consumption of organic solvents and the possibility to automate (off- or on-line) the whole process. LÄS MER

5. 14. Mine the Gaps : Evolution of Eukaryotic Protein Indels and their Application for Testing Deep Phylogeny

   Författare :Pravech Ajawatanawong; Sandra Baldauf; James McInerney; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; indel; insertion deletion; protein evolution; bioinformatics; non-bilateria; eukaryotes; phylogeny; Biology with specialization in Systematics; Biologi med inriktning mot systematik; Biologi med inriktning mot molekylär evolution; Biology with specialization in Molecular Evolution;

   Sammanfattning : Insertions/deletions (indels) are potentially powerful evolutionary markers, but little is known about their evolution and few tools exist to effectively study them. To address this, I developed SeqFIRE, a tool for automated identification and extraction of indels from protein multiple sequence alignments. LÄS MER

7. 15. Interactions between polyphenols and macromolecules facilitate the extraction of low viscosity banana juice

   Författare :Nuria Majaliwa; Chalmers tekniska högskola; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; response surface methodology; polyphenols; banana juice; East African Highland bananas; Fourier Transform Infrared spectroscopy; protein distributions;

   Sammanfattning : Bananas are an important staple food and cash crop throughout the African Great Lakes region where they contribute highly to livelihood and food security. However, bananas are perishable fruits that require effective processing techniques to reduce post-harvest losses. LÄS MER