Sökning: "CRISPR Cas9"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden CRISPR Cas9.

  1. 1. Multifaceted RNA-mediated regulatory mechanisms in Streptococcus pyogenes

    Detta är en avhandling från Umeå : Umeå university

    Författare :Anaïs Le Rhun; Umeå universitet.; Umeå universitet.; Umeå universitet.; [2015]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Streptococcus pyogenes; small RNAs; CRISPR; Cas9; tracrRNA; RNases; gene expression; RNA sequencing; Infectious Diseases; infektionssjukdomar; mikrobiologi; Microbiology;

    Sammanfattning : Bacterial pathogens rely on precise regulation of gene expression to coordinate host infection processes and resist invasion by mobile genetic elements. An interconnected network of protein and RNA regulators dynamically controls the expression of virulence factors using a variety of mechanisms. LÄS MER

  2. 2. Advancing CRISPR technologies to engineer yeast metabolism

    Detta är en avhandling från ; Chalmers tekniska högskola; Gothenburg

    Författare :Raphael Ferreira; [2019]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; oleochemicals; metabolic engineering; Saccharomyces cerevisiae; CRISPR;

    Sammanfattning : The advent of genetic engineering tools has initiated an era of manipulating microorganisms for the production of valuable compounds for our society. Precise engineering of these microbes commonly requires introducing genetic modifications such as gene deletion, overexpression, and accurate regulation in order to enhance the production of the compound of interest. LÄS MER

  3. 3. Optical Mapping of Bacterial Plasmids

    Detta är en avhandling från ; Chalmers tekniska högskola; Gothenburg

    Författare :Vilhelm Müller; [2017]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; competitive binding; optical mapping; fluorescence microscopy; DNA; plasmids; CRISPR Cas9; antibiotic resistance; nanofluidics; single-molecule;

    Sammanfattning : Bacteria that have acquired resistance to antibiotics represent one of the largest threats to human health and modern health care. The genes encoding resistance are frequently spread via the transfer of plasmids, which are circular double stranded DNA molecules separate from the chromosomal DNA of the bacteria. LÄS MER

  4. 4. Glycosaminoglycan Biosynthesis in Zebrafish

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Beata Filipek-Górniok; Uppsala universitet.; [2015]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Heparan sulfate; chondroitin dermatan sulfate; biosynthesis; development; N-deacetylase N-sulfotransferase; glycosyltransferases; morpholino; CRISPR-Cas9;

    Sammanfattning : Proteoglycans (PGs) are composed of highly sulfated glycosaminoglycans chains (GAGs) attached to specific core proteins. They are present in extracellular matrices, on the cell surface and in storage granules of hematopoietic cells. LÄS MER

  5. 5. Functional characterization of the biological significance of the ZBED6/ZC3H11A locus in placental mammals

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Shady Younis; Uppsala universitet.; Uppsala universitet.; [2017]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ZBED6; IGF2; ZC3H11A; Muscle development; Transcriptome analysis; CRISPR Cas9; mRNA export; Molekylär genetik; Molecular Genetics; Bioinformatik; Bioinformatics;

    Sammanfattning : The recent advances in molecular and computational biology have made possible the study of complicated transcriptional regulatory networks that control a wide range of biological processes and phenotypic traits. In this thesis, several approaches were combined including next generation sequencing, gene expression profiling, chromatin and RNA immunoprecipitation, bioinformatics and genome editing methods in order to characterize the biological significance of the ZBED6 and ZC3H11A genes. LÄS MER