Sökning: "protein extraction"

Visar resultat 16 - 20 av 142 avhandlingar innehållade orden protein extraction.

1. 16. Biomarker detection via lab-on-a-chip integrated immunoaffinity approach for fluorescence and mass spectrometry readout

   Författare :Asilah Ahmad Tajudin; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immunoaffinity; affinity prefractionation; immunoMALDI; solid-phase extraction; protein digestion; ISET; antibody microarray; signal amplification; fluorescence; microfluidics; lab on a chip; separation; acoustophoresis; ultrasound; acoustic radiation force; plasma; proteomics; MALDI mass spectrometry; immunoassay; porous silicon.;

   Sammanfattning : The blood/plasma protein biomarker profile represents the pathological and physiological changes relating to human diseases. However, the plasma proteome reflects a high degree of complexity that results in a challenge for most analytical methods in clinical diagnostics. LÄS MER

3. 17. Polypeptide Conjugates as High-affinity Binders for Proteins

   Författare :Lotta Tollstoy Tegler; Lars Baltzer; Amelie Eriksson Karlström; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein recognition; polypeptide conjugates; peptide; molecular interactions; C-reactive protein; Acetylcholine esterase; Human Carboinc anhydrase; Chemistry; Kemi; organisk kemi; Organic Chemistry;

   Sammanfattning : A novel concept for protein recognition has been developed. The recognition unit is a hybrid molecule obtained by conjugation of a small organic molecule to a synthetic polypeptide selected from a 16-membered set of 42 amino acid residue sequences. The sequences are unordered and have no prior relation to the target proteins. LÄS MER

4. 18. The use of fluorescence techniques for the study of some membrane-bound photosynthetic properties and some effects of copper on the thylakoid membrane

   Författare :Göran Samuelsson; N. Valanne; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chlorophylI-protein complexes; sequential extraction; ß-carotene low temperature fluorescence emission; absorption; in vivo chi. a fluorescence; copper; electron transport;

   Sammanfattning : Lyophilized pea chloroplasts were extracted in a stepwise manner in an organic solvent (petroleum ether) with increasing polarity which was obtained by addition of small amount of ETOH (0-1 %). Absorption and low temperature fluorescence emission spectra were measured on both the extracted thylakoids and on isolated chlorophylI-protein complexes. LÄS MER

5. 19. Mass spectrometry in protein structure analysis

   Författare :Andreas Jonsson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Mass spectrometry is an important analytical tool in biological and biochemical research. The speed, accuracy and sensitivity is unmatched by conventional analytical techniques. LÄS MER

7. 20. Assembling a toolkit for computational dissection of dense protein systems

   Författare :Daniel Nilsson; Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; macromolecular crowding; liquid-liquid phase separation; Monte Carlo simulation; time-lagged independent component analysis; finite-size scaling; polymer field theory; protein force field; Fysicumarkivet A:2021:Nilsson;

   Sammanfattning : The cellular interior is a dense environment. Understanding how such an environment impacts the properties of proteins and other macromolecules, as well as how weak, non-specific interactions drive processes such as protein droplet formation through liquid-liquid phase separation, is a major challenge in biological physics. LÄS MER