Sökning: "plasmid"

Visar resultat 1 - 5 av 199 avhandlingar innehållade ordet plasmid.

  1. 1. Novel Chromatography Matrices for Fast Isolation of Plasmid DNA

    Författare :Peter Tiainen; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biokemisk teknik; Polymer technology; biopolymers; Polymerteknik; Biotechnology; Bioteknik; Biochemical technology; Plasmid recovery.; Composite materials; Kompositmaterial; Coating and surface treatment; Skikt och ytbehandling; Superporous agarose; Silica fibres; Plasmid purification; Anion-exchange matrices;

    Sammanfattning : As new therapeutic areas in which genetic material is employed, i.e. gene therapy and DNA vaccination, are expanding, a substantial increase in the demand for gene vectors, e.g. LÄS MER

  2. 2. CopA and CopT: The Perfect RNA Couple

    Författare :Jacoba G. Slagter-Jäger; Gerhart Wagner; Wolfgang Nellen; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microbiology; antisense RNA; plasmid; U-turn; four-way junction; RNA-RNA interaction; Hfq; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Sammanfattning : Antisense RNAs regulate gene expression in many bacterial systems. The best characterized examples are from prokaryotic accessory elements such as phages, plasmids and transposons. Many of these antisense RNAs have been identified as plasmid copy number regulators where they regulate the replication frequency of the plasmid by negative feedback. LÄS MER

  3. 3. Next generation tools for microbial cell factories

    Författare :Alister James Cumming; Daniel Daley; Neil Dixon; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbial cell factories; Recombinant protein production; Bacteria; Plasmid; Genetic sensor; Inducible promoter; Genetic cassette; biokemi; Biochemistry;

    Sammanfattning : The bacterium E. coli is a popular choice for expressing recombinant proteins. It is easy to culture and many molecular tools for are available for directing protein production. When producing recombinant proteins, high yields and good quality soluble products are desirable. LÄS MER

  4. 4. The stochastic nature of intracellular control circuits

    Författare :Johan Paulsson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Stochastic; mesoscopic; noise; gene; plasmid; metabolic; regulation; control; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : Chemical reactions are probabilistic by nature, causing random fluctuations inconcentrations to emerge spontaneously. In conventional (macroscopic) kinetics this internal noise is ignored under the assumption that average copy numbers are so high that fluctuations are negligible in comparison. LÄS MER

  5. 5. Temperature-inducible and calcium-regulated proteins encoded by the virulence plasmid of Yersinia

    Författare :Ingrid Bölin; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Yersinia; virulence plasmid; gene products; regulation; calcium; temperature; outer membrane proteins;

    Sammanfattning : The pathogenic members of the genus Yersinia, Y. pseudotuberculosis, Y. pestis and Y. enterocolitica are transmitted from animals to man and may give rise to disease with a variety of symptoms. LÄS MER