Sökning: "Plasmid purification"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden Plasmid purification.

  1. 1. Novel Chromatography Matrices for Fast Isolation of Plasmid DNA

    Författare :Peter Tiainen; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biokemisk teknik; Polymer technology; biopolymers; Polymerteknik; Biotechnology; Bioteknik; Biochemical technology; Plasmid recovery.; Composite materials; Kompositmaterial; Coating and surface treatment; Skikt och ytbehandling; Superporous agarose; Silica fibres; Plasmid purification; Anion-exchange matrices;

    Sammanfattning : As new therapeutic areas in which genetic material is employed, i.e. gene therapy and DNA vaccination, are expanding, a substantial increase in the demand for gene vectors, e.g. LÄS MER

  3. 2. Purification Processes for Complex Biomacromolecules

    Författare :Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER

  4. 3. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

  5. 4. Production and delivery of recombinant subunit vaccines

    Författare :Christin Andersson; KTH; []
    Nyckelord :Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

    Sammanfattning : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. LÄS MER

  7. 5. Beta-Lactamase in Haemophilus ducreyi: Purification, Characterization, and Mutagenesis

    Författare :Ratana Lawung; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biokemi; Metabolism; Biochemistry; enzyme calorimeter; site-directed mutagenesis; ampicillin resistance plasmid; Haemophilus ducreyi; TEM-1 b-lactamase; metabolism; Proteins; enzymology; Proteiner; enzymologi; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Chancroid caused by Haemophilus ducreyi, has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. Antibiotic resistances are extensively found in H. ducreyi and many of the resistances are due to the presence of resistance plasmids. LÄS MER