Sökning: "ddPCR"

Visar resultat 1 - 5 av 14 avhandlingar innehållade ordet ddPCR.

  1. 1. Molecular mechanism of plasmid copy number control in Yersinia

    Författare :Tifaine Héchard; Helen Wang; Javier Pizarro-Cerda; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Yersinia pseudotuberculosis; plasmid copy number; T3SS; infection; ddPCR; Mikrobiologi; Microbiology;

    Sammanfattning : The ability of pathogenic bacteria to cause disease depends on various virulence mechanisms. The three pathogenic species of Yersinia use a type III secretion system (T3SS) to translocate effector proteins into host cells and disrupt the immune system. This T3SS is encoded on a 70kb, low-copy, virulence plasmid. LÄS MER

  3. 2. Measurable residual disease and clonal evolution in acute myeloid leukemia with focus on NPM1-mutations

    Författare :Louise Pettersson; Lund Patologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; AML; MRD; Measurable residual disease; NPM1; NPM1-mutation; NPM1 type A mutation; clonal evolution; clonal patterns; subclones; genetic evolution; qPCR; RQ-PCR; ddPCR; deep seq; deep sequencing; NGS; RT-qPCR; old; elderly; MFC; flow cytometry;

    Sammanfattning : Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults, with mutations in the NPM1 gene occurring in almost one third of all cases. The ability to detect residual leukemia below the resolution of conventional microscopy is crucial for evaluation of relapse risk after therapy. LÄS MER

  4. 3. Improved methodologies for molecular detection and quantification of viruses in food

    Författare :Sofia Persson; Patrik Ellström; Magnus Simonsson; Wim van der Poel; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; foodborne virus; digital PCR; real-time PCR; validation; assay design; food safety; Medicinsk vetenskap; Medical Science;

    Sammanfattning : Foodborne viruses such as norovirus, hepatitis A virus and hepatitis E virus cause a high burden of disease worldwide. Reverse transcription (RT) quantitative real-time PCR (qPCR) is the current standard method for monitoring viral contamination in the food chain. LÄS MER

  5. 4. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    Författare :Ingrid Ziegler; Kristoffer Strålin; Paula Mölling; Magnus Rasmussen; Örebro universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Sammanfattning : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. LÄS MER

  7. 5. Transmission biology of porcine cysticercosis in eastern-central Tanzania

    Författare :Justine Maganira; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

    Sammanfattning : Porcine cysticercosis (PC) is a neglected zoonotic disease usually acquired by pigs after ingestion of Taenia solium eggs from food or an environment contaminated with human faeces of a pork tapeworm carrier. The disease has serious veterinary and public health implications in low-income endemic countries. LÄS MER