Sökning: "plasmid"

Visar resultat 6 - 10 av 200 avhandlingar innehållade ordet plasmid.

  1. 6. Temperature-inducible and calcium-regulated proteins encoded by the virulence plasmid of Yersinia

    Författare :Ingrid Bölin; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Yersinia; virulence plasmid; gene products; regulation; calcium; temperature; outer membrane proteins;

    Sammanfattning : The pathogenic members of the genus Yersinia, Y. pseudotuberculosis, Y. pestis and Y. enterocolitica are transmitted from animals to man and may give rise to disease with a variety of symptoms. LÄS MER

  3. 7. Control of Chromosome and Plasmid Replication in Escherichia coli

    Författare :Jan Olsson; Kurt Nordström; Santanu Dasgupta; Måns Ehrenberg; Anders Lobner-Olesen; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microbiology; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Sammanfattning : Life is cellular. Cells grow and divide to give two new cells; this process is called the cell cycle. The chromosome in a bacterium is replicated into two identical copies before the cell divides. DNA replication is a fundamental process common to all forms of life. LÄS MER

  4. 8. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

  5. 9. Purification Processes for Complex Biomacromolecules

    Författare :Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER

  7. 10. Production and delivery of recombinant subunit vaccines

    Författare :Christin Andersson; KTH; []
    Nyckelord :Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

    Sammanfattning : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. LÄS MER