Sökning: "antisense RNA"

Visar resultat 1 - 5 av 69 avhandlingar innehållade orden antisense RNA.

  1. 1. CopA and CopT: The Perfect RNA Couple

    Författare :Jacoba G. Slagter-Jäger; Gerhart Wagner; Wolfgang Nellen; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microbiology; antisense RNA; plasmid; U-turn; four-way junction; RNA-RNA interaction; Hfq; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Sammanfattning : Antisense RNAs regulate gene expression in many bacterial systems. The best characterized examples are from prokaryotic accessory elements such as phages, plasmids and transposons. Many of these antisense RNAs have been identified as plasmid copy number regulators where they regulate the replication frequency of the plasmid by negative feedback. LÄS MER

  3. 2. Functional characterization of the small antisense RNA MicA in Escherichia coli

    Författare :Klas Ifeanyi Udekwu; Gerhart Wagner; Tony Romeo; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; small RNA; antisense; outer membrane; Hfq-dependence; RNase III; LuxS; Quorum sensing; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Sammanfattning : The Escherichia coli small RNA (sRNA) MicA was identified recently in a genomewide search for sRNAs. It is encoded between the genes gshA and luxS in E. coli and its close relatives. The function of sRNAs in bacteria is generally believed to be in maintenance of homeostasis via stress-induced modulation of gene expression. LÄS MER

  4. 3. Conformationally Constrained Oligonucleotides for RNA Targeting

    Författare :Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

    Sammanfattning : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. LÄS MER

  5. 4. Small RNA-mediated Regulation of Gene Expression in Escherichia coli

    Författare :Cecilia Unoson; E. Gerhart. H Wagner; Renée Schroeder; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Small RNA; non-coding RNA; antisense; translational control; ribosome standby; toxin-antitoxin; IstR; TisB; tisAB; MicF; Lrp; Microbiology; Mikrobiologi; Cell and molecular biology; Cell- och molekylärbiologi; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Sammanfattning : Non-coding RNAs are highly abundant regulators of gene expression in all kingdoms of life that often play important roles in vital cellular functions. In bacteria, small regulatory RNAs (sRNAs) usually act post-transcriptionally by regulating mRNAs through base pairing within ribosome binding sites (RBS), thereby inhibiting translation initiation. LÄS MER

  7. 5. Making Sense of Antisense

    Författare :Johan Reimegård; Gerhart Wagner; Hanah Margalit; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; sRNA; miRNA; RNA; target prediction; SOLiD siRNA; E.coli; D.discoideum; Biology; Biologi; Bioinformatics; Bioinformatik; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : RNA is a highly versatile molecule with functions that span from being a messenger in the transfer from DNA to protein, a catalytic molecule important for key processes in the cell to a regulator of gene expression. The post-genomic era and the use of new techniques to sequence RNAs have dramatically increased the number of regulatory RNAs during the last decade. LÄS MER