Sökning: "histidine"

Visar resultat 21 - 25 av 141 avhandlingar innehållade ordet histidine.

  1. 21. Studies on Phosphohistidine Phosphatase 1 : What? Where? Why?

    Författare :Ulla Beckman Sundh; Pia Ek; Örjan Zetterqvist; Jin-ping Li; Christian Hedberg; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; PHPT1; Phosphohistidine phosphatase; PHP; Assay; Medical Biochemistry; Medicinsk biokemi;

    Sammanfattning : Phosphohistidine phosphatase 1 (PHPT1) is a small protein, consisting of 125 amino acids, that catalyzes the dephosphorylation of histidine but does not have any activity towards other phosphorylated amino acids. PHPT1 was identified in 2002, and is so far the only mammalian histidine phosphatase known, but still little is known about its physiological role. LÄS MER

  3. 22. Protein phosphorylation in plant mitochondria

    Författare :André Struglics; Lund Ortopedi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FoF1-ATPase; autophosphorylation; protein kinase activity; protein phosphorylation; Plant mitochondria; inner mitochondrial membrane; NDPK; Physiological biophysics; Växtfysiologi;

    Sammanfattning : Protein phosphorylation in the subcompartments of plant mitochondria was investigated by labelling with [gamma-32P]ATP and by SDS-PAGE/autoradiography. About 20 proteins in inside-out inner mitochondrial membranes from potato tubers were phosphorylated by endogenous protein kinases when incubated with [gamma-32P]ATP. LÄS MER

  4. 23. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Författare :Florent Bernaudat; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER

  5. 24. A radical view of haemoglobin - protein engineering and characterisation

    Författare :Marie Grey; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

    Sammanfattning : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. LÄS MER

  7. 25. Development of ADAPT-based tracers for radionuclide molecular imaging of cancer

    Författare :Javad Garousi; Vladimir Tolmachev; Tony Lahoutte; Uppsala universitet; []
    Nyckelord :ADAPT; scaffold protein; albumin-binding domain ABD ; Affinity protein; HER2; radionuclide molecular imaging;

    Sammanfattning : ABD-Derived Affinity Proteins (ADAPTs) is a novel class of small engineered scaffold proteins based on albumin-binding domain (ABD) of streptococcal protein G. High affinity ADAPT  binders against various therapeutic targets can be selected. LÄS MER