Sökning: "Site directed mutagenesis"

Visar resultat 21 - 25 av 150 avhandlingar innehållade orden Site directed mutagenesis.

  1. 21. Plastidial phosphate transport in plants

    Författare :Patrik Milton Karlsson; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Arabidopsis; phosphate transport; thylakoid membrane; root plastid; photosynthesis; leaf size; starch metabolism; homology modeling; site-directed mutagenesis;

    Sammanfattning : Phosphorus is an essential element for all living organisms and is central to the genetics and energetics of life. Inorganic phosphate (Pi) is recurrently involved in protein regulation and signal transduction but also in energy transfer as a component of the ATP-molecule. LÄS MER

  3. 22. The structural basis for the catalytic specificity of manganese lipoxygenases : 3D structure analysis of the lipoxygenase of Magnaporthe oryzae

    Författare :Anneli Wennman; Ernst H. Oliw; Marcia Newcomer; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; oxylipin; lipoxygenase; crystal structure; crystallography; HPLC; mass spectrometry; yeast; site-directed mutagenesis; fungi; Biochemical Pharmacology; Biokemisk farmakologi;

    Sammanfattning : Lipoxygenases (LOX) catalyze regio- and stereospecific oxygenation of polyunsaturated fatty acids to hydroperoxides. These hydroperoxides are further metabolized to leukotrienes and lipoxins in mammals, and are involved in asthma and inflammation. LOX of animals and plants contain iron as catalytic metal (FeLOX). LÄS MER

  4. 23. Structure-function relationships of hormone-sensitive lipase

    Författare :Torben Österlund; Institutionen för translationell medicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proteins; mutagenesis; domain; fold; catalytic triad; lipase; structure; enzymology; Proteiner; enzymologi;

    Sammanfattning : The primary role of hormone-sensitive lipase (HSL), an 84 kDa enzyme of 768 amino acids (in the rat protein), is to hydrolyse stored triacylglycerols in adipocytes of white adipose tissue. Catecholamines and insulin regulate lipolysis through cellular signalling. LÄS MER

  5. 24. Elongation factor 2: A key component of the translation machinery in eukaryotes : Properties of yeast elongation factor 2 studied in vivo

    Författare :Galyna Bartish; Odd Nygård; Suparna Sanyal; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Elongation factor 2; yeast; ribosome; phosphorylation; Switch I; site-directed mutagenesis; functional complementation; Cell biology; Cellbiologi; cellbiologi; Cellbiology;

    Sammanfattning : Synthesis of proteins is performed by the ribosome, a large ribonucleoprotein complex. Apart from the ribosome, numerous protein factors participate in this process. Elongation factor 2 (eEF2) is one of these factors. eEF2 is an essential protein with a mol. LÄS MER

  7. 25. Escherichia coli transhydrogenase : an NMR and crystallisation study

    Författare :Tomas Johansson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; paramagnetic NMR; site-directed mutagenesis; structure function; transhydrogenase; srystallisation; protein-protein interactions; membrane protein;

    Sammanfattning : .... LÄS MER