Sökning: "Site directed mutagenesis"

Visar resultat 6 - 10 av 150 avhandlingar innehållade orden Site directed mutagenesis.

  1. 6. Beta-Lactamase in Haemophilus ducreyi: Purification, Characterization, and Mutagenesis

    Författare :Ratana Lawung; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biokemi; Metabolism; Biochemistry; enzyme calorimeter; site-directed mutagenesis; ampicillin resistance plasmid; Haemophilus ducreyi; TEM-1 b-lactamase; metabolism; Proteins; enzymology; Proteiner; enzymologi; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Chancroid caused by Haemophilus ducreyi, has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. Antibiotic resistances are extensively found in H. ducreyi and many of the resistances are due to the presence of resistance plasmids. LÄS MER

  3. 7. Expression and Mutagenesis studies of Candida antactica lipase B

    Författare :Johanna C. Rotticci-Mulder; KTH; []
    Nyckelord :Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER

  4. 8. Study on the Function of Translation Initiation Factor IF1

    Författare :Victor Croitoru; Leif A. Isaksson; Kim Kusk Mortensen; Stockholms universitet; []
    Nyckelord :translation; initiation factor IF1; mutagenesis; A-site; RNA chaperone; NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : Initiation is the first step in protein biosynthesis representing a fundamental event in cell life which determines fidelity, efficiency and regulation of gene expression. In addition to the ribosome and mRNA, three protein factors IF1, IF2 and IF3 are involved in the initiation of translation in prokaryotes. LÄS MER

  5. 9. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis

    Författare :Wei Zhang; Bengt Mannervik; Bengt-Harald Jonsson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; azathioprine; directed evolution; semi-rational design; catalytic mechanism; saturation mutagenesis; reduced amino acid alphabets; molecular docking; protein evolution; multivariate data analysis; epistasis; fitness landscape; evolutionary trajectories; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. LÄS MER

  7. 10. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

    Författare :Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

    Sammanfattning : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. LÄS MER