Sökning: "K. lactis"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden K. lactis.

  1. 1. Mating type switching and transcriptional silencing in Kluyveromyces lactis

    Författare :Emad Barsoum; Åström Stefan; Laura Rusche; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mating type switch; transcriptional silencing; alpha3; transposase; Ume6; Mts1; Ras; cAMP; K. lactis; Developmental biology; Utvecklingsbiologi; utvecklingsbiologi; Developmental Biology;

    Sammanfattning : To explore the similarities and differences of regulatory circuits among budding yeasts, we characterized the role of unscheduled meiotic gene expression 6 (UME6) and a novel mating type switching pathway in Kluyveromyces lactis. We found that Ume6 was required for transcriptional silencing of the cryptic mating-type loci HMLα and HMRa. LÄS MER

  3. 2. The Kluyveromyces lactis killer toxin is a transfer RNA endonuclease

    Författare :Jian Lu; Anders Byström; Leif Kirsebom; Umeå universitet; []
    Nyckelord :Molecular biology; K. lactis γ-toxin; tRNA endonuclease; modified nucleosides; 5-methoxycarbonylmethyl-2-thiouridine; 5-methylaminomethyl-2-thiouridine; anticodon stem loop; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : Killer strains of the yeast Kluyveromyces lactis secrete a heterotrimeric protein toxin (zymocin) to inhibit the growth of sensitive yeasts. The cytotoxicity of zymocin resides in the γ subunit (γ-toxin), however the mechanism of cytotoxicity caused by γ-toxin was previously unknown. LÄS MER

  4. 3. Regulation and mechanism of mating-type switching in Kluyveromyces lactis

    Författare :Naghmeh Rajaei; Stefan Åström; Kenneth Wolfe; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; yeast; mating-type switching; DSB; gene conversion; transposable elements; RAS cAMP pathway; nutrient limitation; molekylär biovetenskap; Molecular Bioscience;

    Sammanfattning : Transposable elements (TEs) have had immense impact on the structure, function and evolution of eukaryotic genomes. The work in this thesis identified Kat1, a novel domesticated DNA transposase of the hAT family in the yeast Kluyveromyces lactis. Kat1 triggers a genome rearrangement that results in a switch of mating type from MATa to MATα. LÄS MER

  5. 4. Characterization of Budding Yeast Nonhomologous End-Joining at DNA Double-Strand Breaks and Telomeres

    Författare :Sidney Carter; Stefan Åström; Ed Louis; Stockholms universitet; []
    Nyckelord :DNA repair; nonhomologous end-joining; double-strand break; Developmental Biology; miljömedicinsk utvecklingsbiologi;

    Sammanfattning : The yeast K. lactis efficiently integrates DNA by illegitimate recombination (IR). IR was completely dependent upon nonhomologous end-joining (NHEJ). In contrast to S. LÄS MER

  7. 5. Silencing and DNA double-strand break repair in budding yeast

    Författare :Andreas Kegel; Stefan Åström; Stephan Marcand; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Silencing; Reb1; DSB; NHEJ; Nej1; illegitimate recombination; yeast; Developmental biology; Utvecklingsbiologi;

    Sammanfattning : Transcriptional silencing that makes large chromosomal domains inaccessible for the transcriptional apparatus is nucleated at DNA elements called silencers. In K.lactis a 102bp HMLα silencer was defined revealing three distinct protein-binding regions (A, B, and C) that were required for silencing of HMLα. LÄS MER