Sökning: "Hydrogen Deuterium Exchange Mass Spectrometry"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden Hydrogen Deuterium Exchange Mass Spectrometry.

1. 1. Capillary electrophoresis mass spectrometry applied to structural proteomics and small molecule analysis

   Författare :Jordan Aerts; Erik T. Jansson; Per E. Andrén; Petr Man; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary Electrophoresis Mass Spectrometry; Hydrogen Deuterium Exchange Mass Spectrometry; Enantioselective Separations; Drugs of Abuse; Analytical Chemistry; Analytisk kemi; Analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

   Sammanfattning : Capillary electrophoresis with mass spectrometric (CE–MS) detection offers a separation method without equal in terms of flexibility, utility, and cost efficiency. Here we demonstrate precisely this through the application of several laboratory-built CE–MS instruments for the separation of brain metabolites in non-primates, enantioselective separations of synthetic anesthetic metabolites in fractionated pony urine, application in structural proteomics workflows, and identification of exogenous alkaloid biotransformationproducts in human cerebrospinal fluid (CSF). LÄS MER

3. 2. Method Development in Quantitative and Structural Proteomics using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Charlotte Hagman; Per Håkansson; Jonas Bergquist; Jos Buijs; Michael Przybylski; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Cerebrospinal Fluid; Electrospray Ionization; Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Global Markers; High Performance Liquid Chromatography; Gas-Phase Exchange; Hydrogen Deuterium Exchange; Plasma; Protoemics; Quantification; Structural Elucidation; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : In this thesis, methods for studying different aspects of proteomics were developed with Fourier Transform Ion Cyclotron Resonance, (FTICR), mass spectrometry. The FTICR technique provides ultra-high mass resolving power, mass accuracy at sub ppm level and sensitivity in the attomole region. LÄS MER

4. 3. Method and technique development in peptide and protein mass Spectrometry

   Författare :Kristina Håkansson; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; electrospray ionization; peptide sequencing; Fourier transform ion cyclotron resonance; hydrogen deuterium exchange; protein structure; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Mechanistic studies and applicability of several methods and techniques for studying peptides and proteins with mass spectrometry are presented. A model for the protein molecular ion formation in explosive matrix-assisted plasma desorption mass spectrometry is proposed, involving a contribution from the chemical energy release during conversion of the explosive, an enhanced protonation through collisions with products from the explosive decay, and electron scavenging by other products. LÄS MER

5. 4. Advanced computations and mass-spectrometric techniques to unravel the dynamics and interactions of proteins

   Författare :Joana Costeira-Paulo; Erik Marklund; Lars Konermann; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; molecular dynamics; mass spectrometry; dihydroorotate dehydrogenase; alpha-synuclein; protein structure; ion mobility; electrospray ionisation; collision cross section; lipids; gas-phase hydrogen deuterium exchange; collision induced unfolding; Biokemi; Biochemistry;

   Sammanfattning : Proteins are an important class of biomolecules, involved in the metabolism, regulation, structure and transport in cells. Unfortunately, many diseases are caused by protein dysfunction. When proteins perform their normal function, they may interact with each other or change their conformation. LÄS MER

7. 5. Macromolecules at Interfaces

   Författare :Helén Larsericsdotter; Sven Oscarsson; Jos Buijs; Willem Norde; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; Protein; Adsorption; Immobilization; Surface; Surface Plasmon Resonance; SPR; Mass Spectrometry; MS; Differential Scanning Calorimetry; DSC; FT ICR; Lysozyme; Bovine Serum Albumin; BSA; Myoglobin; Globular; Kemi; Chemistry; Kemi;

   Sammanfattning : In this thesis, the structure and stability of globular proteins adsorbed onto nanometer-sized hydrophilic silica particles were investigated using differential scanning calorimetry (DSC), hydrogen/deuterium exchange (HDX), and mass spectrometry (MS). The adsorption process itself was characterized with fluorescence and absorption spectroscopy and surface plasmon resonance (SPR). LÄS MER