Sökning: "peptide sequencing"

Visar resultat 1 - 5 av 68 avhandlingar innehållade orden peptide sequencing.

1. 1. Identification and Characterization of Peptides and Proteins using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Magnus Palmblad; Ole Nørregaard Jensen; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; Peptide; protein; peptide mass fingerprinting; identification; sequencing; protein structure; non-covalent interaction; modification; electrospray ionization; liquid chromatography; capillary electrophoresis; electron capture dissociation; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyloid β-peptide; Alzheimer’s disease.; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Mass spectrometry has in recent years been established as the standard method for protein identification and characterization in proteomics with excellent intrinsic sensitivity and specificity. Fourier transform ion cyclotron resonance is the mass spectrometric technique that provides the highest resolving power and mass accuracy, increasing the amount of information that can be obtained from complex samples. LÄS MER

3. 2. Method and technique development in peptide and protein mass Spectrometry

   Författare :Kristina Håkansson; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; electrospray ionization; peptide sequencing; Fourier transform ion cyclotron resonance; hydrogen deuterium exchange; protein structure; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Mechanistic studies and applicability of several methods and techniques for studying peptides and proteins with mass spectrometry are presented. A model for the protein molecular ion formation in explosive matrix-assisted plasma desorption mass spectrometry is proposed, involving a contribution from the chemical energy release during conversion of the explosive, an enhanced protonation through collisions with products from the explosive decay, and electron scavenging by other products. LÄS MER

4. 3. Chlorite dismutase of ideonella dechloratans

   Författare :Helena Danielsson Thorell; KTH; []
   Nyckelord :Chlorite dismutase; Ideonella dechloratans; Purification; Genomic; Library; Gene; PCR DNA; Sequencing; Signal peptide;

   Sammanfattning : .... LÄS MER

5. 4. New Proteomics Methods and Fundamental Aspects of Peptide Fragmentation

   Författare :Mikhail Savitski; Roman Zubarev; Per Andren; David Fenyö; Uppsala universitet; []
   Nyckelord :Bioinformatics; Proteomics; Peptide fragmentation; Bioinformatik;

   Sammanfattning : The combination of collision-activated dissociation, (CAD) and electron capture dissociation, (ECD) yielded a 125% increase in protein identification. The S-score was developed for measuring the information content in MS/MS spectra. This measure made it possible to single out good quality spectra that were not identified by a search engine. LÄS MER

7. 5. Array-based Autoantibody Profiling and Epitope Mapping

   Författare :Arash Zandian; Peter Nilsson; Jochen M. Schwenk; Maja Neiman; Morten Nielsen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antigens; peptide; epitope mapping; autoimmunity; autoantibodies; microarrays; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies are a class of proteins that are made by the immune system to recognize harmful organisms and molecules. Their exceptional capability of specifically recognizing molecules has been investigated for over a century and information thereof has been utilized for a variety of applications including vaccine and generation of therapeutic antibodies. LÄS MER