Sökning: "GH5"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet GH5.

  1. 1. Exploring glycoside hydrolase family 5 (GH5) enzymes

    Författare :Yang Wang; Ines Ezcurra; Henrik Aspeborg; Henrik Stålbrand; Torkel Berglund; KTH; []
    Nyckelord :GH5; glycoside hydrolase; subfamily classification; mannans; polysaccharides; mannanases; cell wall; GH5; glykosidhydrolas; subfamiljklassificering; mannaner; polysackarider; mannanaser; cellvägg; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : In 1990, the classification of carbohydrate-active enzymes (CAZymes) was introduced by the scientist Bernard Henrissat. According to sequence similarity, these enzymes were separated into families with conserved structures and reaction mechanisms. LÄS MER

  3. 2. β-Mannan degradation by gut bacteria - Characterization of β-mannanases from families GH5 and GH26

    Författare :Evelina Kulcinskaja; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; gut microflora; mannan; enzymes; GH26; GH5; β-mannanase;

    Sammanfattning : The human gut flora is important for our well-being. The gut bacteria are able to degrade and metabolize complex carbohydrates. Examples of such carbohydrates are β-mannans. β-Mannans consist of a backbone of β-1,4-linked mannose units and are present in e. LÄS MER

  4. 3. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Författare :Johan Morrill; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Sammanfattning : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. LÄS MER

  5. 4. Discovery and investigation of glycoside hydrolase family 5 enzymes with potential use in biomass conversion

    Författare :Yang Wang; Henrik Aspeborg; Ines Ezcurra; Jerry Ståhlberg; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; plant cell wall; biomass; hemicellulose; mannans; glycoside hydrolase; subfamily classification; Arabidopsis; mannanase; transglycosylase; enzymatic characterization.; Bioteknologi; Biotechnology;

    Sammanfattning : Glycoside hydrolases (GHs) cleave glycosidic bonds in glycoconjugates, oligosaccharides and polysaccharides such as cellulose and various hemicelluloses. Mannan is a major group of hemicelluloses. LÄS MER

  7. 5. Valorisation of cereal by-products: a biorefinery approach

    Författare :Reskandi C. Rudjito; Franscisco Vilaplana; Amparo Jimenez-Quero; Mirjam Kabel; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Cereal by-products; arabinoxylan; ferulic acid; subcritical water extraction; enzymatic treatment; xylanases; prebiotics; Jordbruksbiprodukter; arabinoxylan; ferulsyra; subkritisk vattenextraktion; enzymatisk behandling; xylanaser; prebiotika; Bioteknologi; Biotechnology;

    Sammanfattning : Cereal by-products are abundant streams of underutilised biomass that have the potential be valorised into various industrial products by means of environmentally friendly processes. In this study, an integrated bioprocess combining subcritical water extraction (SWE) and enzymatic treatment (ET) was proposed to effectively extract arabinoxylans (AX) from cereal by-products. LÄS MER