Sökning: "mannanases"

Visar resultat 1 - 5 av 13 avhandlingar innehållade ordet mannanases.

  1. 1. Exploring glycoside hydrolase family 5 (GH5) enzymes

    Författare :Yang Wang; Ines Ezcurra; Henrik Aspeborg; Henrik Stålbrand; Torkel Berglund; KTH; []
    Nyckelord :GH5; glycoside hydrolase; subfamily classification; mannans; polysaccharides; mannanases; cell wall; GH5; glykosidhydrolas; subfamiljklassificering; mannaner; polysackarider; mannanaser; cellvägg; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : In 1990, the classification of carbohydrate-active enzymes (CAZymes) was introduced by the scientist Bernard Henrissat. According to sequence similarity, these enzymes were separated into families with conserved structures and reaction mechanisms. LÄS MER

  3. 2. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Författare :Johan Morrill; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Sammanfattning : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. LÄS MER

  4. 3. β-Mannan degradation by gut bacteria - Characterization of β-mannanases from families GH5 and GH26

    Författare :Evelina Kulcinskaja; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; gut microflora; mannan; enzymes; GH26; GH5; β-mannanase;

    Sammanfattning : The human gut flora is important for our well-being. The gut bacteria are able to degrade and metabolize complex carbohydrates. Examples of such carbohydrates are β-mannans. β-Mannans consist of a backbone of β-1,4-linked mannose units and are present in e. LÄS MER

  5. 4. Structure and Function of Beta-Mannanases from Glycoside Hydrolase Clan A. A Study of Hemicellulases from Microbes and a Mollusc

    Författare :Lars Anderson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; Proteins; subsite organisation; transglycosylation; enzyme kinetics; beta-mannanase; hemicellulose;

    Sammanfattning : Hemicellulose is the second most abundant group of renewable polysaccharides. Mannans can be found as the major hemicellulose in softwood and as storage polysaccharides in a range of plants. In this work, structural and functional characterisations of family 5 and 26 endo-1,4-b-mannanases (b-mannanases) have been performed. LÄS MER

  7. 5. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

    Författare :Anna Rosengren; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

    Sammanfattning : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. LÄS MER