Sökning: "β-mannanase"

Visar resultat 1 - 5 av 10 avhandlingar innehållade ordet β-mannanase.

  1. 1. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Författare :Johan Morrill; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Sammanfattning : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. LÄS MER

  3. 2. β-Mannan degradation by gut bacteria - Characterization of β-mannanases from families GH5 and GH26

    Författare :Evelina Kulcinskaja; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; gut microflora; mannan; enzymes; GH26; GH5; β-mannanase;

    Sammanfattning : The human gut flora is important for our well-being. The gut bacteria are able to degrade and metabolize complex carbohydrates. Examples of such carbohydrates are β-mannans. β-Mannans consist of a backbone of β-1,4-linked mannose units and are present in e. LÄS MER

  4. 3. galactmannan degradation by fungi and gut bacteria : structural enzymology and fine -tuned substrate specifcity

    Författare :Sumitha Krishnaswamyreddy; Biokemi och Strukturbiologi; []
    Nyckelord :fine-tuned substrate specificity; β-mannanase; β-mannosidases; synergy; α-galactosidases; mannan converison in gut; transglycosylation; polysaccharide utilisation locus; phylogenetic analysis; MALDI-TOF MS;

    Sammanfattning : AbstractThe degradation of plant based β-mannan polysaccharides represents one of the many challenges efficiently tackled by microorganisms living in different habitats. In this thesis, glycoside hydrolases (GHs) involved in mannan degradation from two different organisms, Aspergillus nidulans (paper I and II) and Bacteroides ovatus (paper III and IV) were studied. LÄS MER

  5. 4. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

    Författare :Anna Rosengren; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

    Sammanfattning : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. LÄS MER

  7. 5. Enzymatic conversion of β-mannans through transglycosylation and hydrolysis : Synthesis of building blocks for novel biomaterials from renewables

    Författare :Samuel Butler; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-Mannanase; Transglycosylation; Enzymatic synthesis; Glycoconjugates; Enzyme synergy; α- Galactosidase; Galactoglucomannan; Enzyme engineering; MALDI-ToF MS; HPLC;

    Sammanfattning : The major softwood hemicellulose is galactoglucomannan (GGM), an abundant polysaccharide which constitutes up to 25% of the dry weight of wood, much of which is discarded in current refinery practices. Among the enzymes responsible for the degradation of this hemicellulose are the β-mannanases. LÄS MER