Sökning: "β-mannanase"

Visar resultat 1 - 5 av 7 avhandlingar innehållade ordet β-mannanase.

  1. 1. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Detta är en avhandling från Lund University, Faculty of Science, Department of Chemistry, Division of Biochemistry and Structural Biology

    Författare :Johan Morrill; [2017]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Sammanfattning : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. LÄS MER

  2. 2. β-Mannan degradation by gut bacteria - Characterization of β-mannanases from families GH5 and GH26

    Detta är en avhandling från Department of Chemistry, Lund University

    Författare :Evelina Kulcinskaja; [2015]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; gut microflora; mannan; enzymes; GH26; GH5; β-mannanase;

    Sammanfattning : The human gut flora is important for our well-being. The gut bacteria are able to degrade and metabolize complex carbohydrates. Examples of such carbohydrates are β-mannans. β-Mannans consist of a backbone of β-1,4-linked mannose units and are present in e. LÄS MER

  3. 3. galactmannan degradation by fungi and gut bacteria : structural enzymology and fine -tuned substrate specifcity

    Detta är en avhandling från Lund University, Faculty of Science, Department of Chemistry, Division of Biochemistry and Structural Biology

    Författare :Sumitha Krishnaswamyreddy; [2016]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fine-tuned substrate specificity; β-mannanase; β-mannosidases; synergy; α-galactosidases; mannan converison in gut; transglycosylation; polysaccharide utilisation locus; phylogenetic analysis; MALDI-TOF MS;

    Sammanfattning : AbstractThe degradation of plant based β-mannan polysaccharides represents one of the many challenges efficiently tackled by microorganisms living in different habitats. In this thesis, glycoside hydrolases (GHs) involved in mannan degradation from two different organisms, Aspergillus nidulans (paper I and II) and Bacteroides ovatus (paper III and IV) were studied. LÄS MER

  4. 4. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

    Detta är en avhandling från Department of Chemistry, Lund University

    Författare :Anna Rosengren; [2014]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

    Sammanfattning : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. LÄS MER

  5. 5. Endoglucanase and Mannanase from Blue Mussel, Mytilus edulis: Purification, Characterization, Gene and Three Dimensional Structure

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Bingze Xu; Uppsala universitet.; [2002]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Bioteknik; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik; Surface Biotechnology; ytbioteknik;

    Sammanfattning : Two polysaccharide-degrading enzymes (endo-1,4-D-glucanase and β-mannanase) from blue mussel, Mytilus edulis, have been purified to homogeneity using a combination of several chromatographic steps. Each enzyme has been characterized with regard to its molecular weight, isoelectric point, pH and temperature stability, pH and temperature optimum and substrate specificity. LÄS MER