Sökning: "GH26"

Visar resultat 1 - 5 av 6 avhandlingar innehållade ordet GH26.

1. 1. β-Mannan degradation by gut bacteria - Characterization of β-mannanases from families GH5 and GH26

   Författare :Evelina Kulcinskaja; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; gut microflora; mannan; enzymes; GH26; GH5; β-mannanase;

   Sammanfattning : The human gut flora is important for our well-being. The gut bacteria are able to degrade and metabolize complex carbohydrates. Examples of such carbohydrates are β-mannans. β-Mannans consist of a backbone of β-1,4-linked mannose units and are present in e. LÄS MER

3. 2. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

   Författare :Johan Morrill; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

   Sammanfattning : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. LÄS MER

4. 3. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

   Författare :Anna Rosengren; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

   Sammanfattning : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. LÄS MER

5. 4. The digestive machinery of a human gut bacterium : Structural enzymology of galactomannan utilisation

   Författare :Viktoria Bågenholm; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; Glycoside hydrolase; enzymology; structural biology; kinetics; HPLC; product profiles; structure-function;

   Sammanfattning : Human gut bacteria utilise different types of polysaccharides present in our diet. One of these polysaccharides is galactomannan. Many organisms in the phylum Bacteroidetes have gene clusters encoding for all proteins required for hydrolysis, binding and transport of one type of polysaccharide, called polysaccharide utilisation loci (PULs). LÄS MER

7. 5. galactmannan degradation by fungi and gut bacteria : structural enzymology and fine -tuned substrate specifcity

   Författare :Sumitha Krishnaswamyreddy; Biokemi och Strukturbiologi; []
   Nyckelord :fine-tuned substrate specificity; β-mannanase; β-mannosidases; synergy; α-galactosidases; mannan converison in gut; transglycosylation; polysaccharide utilisation locus; phylogenetic analysis; MALDI-TOF MS;

   Sammanfattning : AbstractThe degradation of plant based β-mannan polysaccharides represents one of the many challenges efficiently tackled by microorganisms living in different habitats. In this thesis, glycoside hydrolases (GHs) involved in mannan degradation from two different organisms, Aspergillus nidulans (paper I and II) and Bacteroides ovatus (paper III and IV) were studied. LÄS MER