Sökning: "Enzyme Activation"

Visar resultat 1 - 5 av 366 avhandlingar innehållade orden Enzyme Activation.

  1. 1. Rational engineering of esterases for improved amidase specificity in amide synthesis and hydrolysis

    Författare :Peter Hendil-Forssell; Mats Martinelle; Romas J. Kazlauskas; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Amidase; Biocatalysis; Enzyme; Esterase; Enzyme engineering; Lipase; Substrate specificity; Biotechnology; Bioteknologi;

    Sammanfattning : Biocatalysis is an ever evolving field that uses enzymes or microorganisms for chemical synthesis. By utilizing enzymes that generally have evolved for specific reactions under mild conditions and temperatures, biocatalysis can be a more environmentally friendly option compared to traditional chemistry. LÄS MER

  2. 2. Enzyme Cold Adaptation through Evolution of Protein Flexibility

    Författare :Jaka Sočan; Johan Åqvist; Hugo Gutiérrez-de-Terán; Janez Mavri; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; EVB; Empirical valence bond method; Pancreatic elastase; α-amylase; Enzyme catalysis; Enzyme evolution; Cold adaptation; Temperature dependence of catalytic rates; Psychrophilic enzyme; Molekylär biovetenskap; Molecular Life Sciences;

    Sammanfattning : What lives, evolves. Macromolecular catalysis is a process, central to both evolutionary and metabolic aspect of life, as it provides a systematic bias favouring certain chemical processes. LÄS MER

  3. 3. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity

    Författare :Olof Modén; Bengt Mannervik; Mikael Widersten; Kenneth Tew; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; allelic variants; azathioprine; bioactivation; chimeric mutagenesis; directed evolution; DNA shuffling; enzyme engineering; glutathione transferase; GST; lysate screening; molecular docking; multiple alignment; multivariate analysis; polymorphism; principal component analysis; prodrug; prodrug activation; protein engineering; protein redesign; reduced amino acid alphabet; saturation mutagenesis; semi-rational enzyme engineering; site-directed mutagenesis; structure-activity relationship; structure-based redesign; Biokemi; Biochemistry;

    Sammanfattning : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. LÄS MER

  4. 4. Enzyme Immobilization in Mesoporous Silica

    Författare :Hanna Gustafsson; Chalmers University of Technology; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ENGINEERING AND TECHNOLOGY; immobilization; lipase; Mesoporous; particle morphology; feruloyl esterase; enzyme; encapsulation; silica; pore size; trypsin;

    Sammanfattning : Catalysts are widely used in many industries for production of chemicals, pharmaceuticals, fuels and energy. There is a need for more sustainable production processes, with reduced energy and raw material consumption together with a reduction in waste and toxic by-products. LÄS MER

  5. 5. Trypsinogen and Its Activation in Acute Pancreatitis

    Författare :Ulf Petersson; Kirurgi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TAP; ERCP; carboxypeptidase B activation peptide; trypsinogen activation peptide; trypsinogen activation; Acute pancreatitis; trypsinogen; CAPAP; Surgery; orthopaedics; traumatology; Kirurgi; ortopedi; traumatologi;

    Sammanfattning : Pancreatic enzymes, neutrophils, free radicals and cytokines are involved in the pathophysiology of AP. Clarification of their interaction and time-course will improve our understanding of AP and provide a better basis for treatment. LÄS MER