Sökning: "Emma Lundberg"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden Emma Lundberg.

  1. 1. Bioimaging for analysis of protein expression in cells and tissues using affinity reagents

    Författare :Emma Lundberg; Torbjörn Gräslund; Mathias Uhlén; Rainer Pepperkok; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody; antibody; biomarker; cell line; cell microarray; colorectal cancer; confocal microscopy; HER2; immunohistochemistry; immunofluorescence; light microscopy; protein expression; protein localization; SATB2; tissue microarray; Bioengineering; Bioteknik; Cell and molecular biology; Cell- och molekylärbiologi;

    Sammanfattning : The detection and analysis of biomolecules, such as proteins, are of great interest since these molecules are fundamental for life and our health. Due to the complexity of biological processes, there is a great advantage of studying proteins in their natural context, for example by using bioimaging. LÄS MER

  2. 2. Integration of RNA and protein expression profiles to study human cells

    Författare :Frida Danielsson; Emma Lundberg; Mikael Huss; Mathias Uhlén; Christine Vogel; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; RNA-seq; Transcriptomics; Proteomics; Malignant transformation; Cancer; Functional enrichment; Bioteknologi; Biotechnology; Medical Technology; Medicinsk teknologi;

    Sammanfattning : Cellular life is highly complex. In order to expand our understanding of the workings of human cells, in particular in the context of health and disease, detailed knowledge about the underlying molecular systems is needed. LÄS MER

  3. 3. Spatiotemporal characterization of the human proteome

    Författare :Diana Mahdessian; Emma Lundberg; Arne Lindqvist; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Spatial proteomics; Spatiotemporal proteomics; Immunofluorescence; Human Protein Atlas; Cell compartments; Single cell proteomics; Cell cycle; Cancer;

    Sammanfattning : Characterizing the molecular components of the basic unit of life; the cell, is crucial for a complete understanding of human biology. The cell is divided into compartments to create a suitable environment for the resident proteins to fulfill their functions. LÄS MER

  4. 4. Antibody-based subcellular localization of the human proteome

    Författare :Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. LÄS MER

  5. 5. Towards subcellular localization of the human proteome using bioimaging

    Författare :Charlotte Stadler; Emma Lundberg; Carolina Wählby; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

    Sammanfattning : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. LÄS MER