Sökning: "Torbjörn Gräslund"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden Torbjörn Gräslund.

  1. 1. Protein Engineering by Directed Evolution and Rational Design

    Författare :Torbjörn Gräslund; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ion-exchange chromatography; subtilisin; Klenow DNA polymerase; Taq DNA polymerase; directed evolution; rational design; charge engineering; phosphonylating inhibitor; phage display; circular dichroism; protein A; Bioengineering; Bioteknik;

    Sammanfattning : .... LÄS MER

  2. 2. Tools in biocatalysis : enzyme immobilisation on silica and synthesis of enantiopure amines

    Författare :Karim Engelmark Cassimjee; Per Berglund; Torkel Berglund; Torbjörn Gräslund; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : This thesis presents two techniques in the field of biocatalysis: An enzyme immobilisation method based on the His6-tag for attachment on modified silica oxide beads, and it’s employment in aqueous and organic medium for synthesis applications. The method functions as a one step extraction and immobilisation protocol. LÄS MER

  3. 3. Biochemical and structural characterization of two thermostable carbohydrate-active enzymes with potential in biotechnological applications

    Författare :Noor Hassan; Christina Divne; Torbjörn Gräslund; Henrik Hansson; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : .... LÄS MER

  4. 4. Engineering of Affibody molecules for Radionuclide Molecular Imaging and Intracellular Targeting

    Författare :Camilla Hofström; Torbjörn Gräslund; Serge Muyldermans; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; Affibody molecules; affinity proteins; radionuclide molecular imaging; intracellular targeting; EGFR; HER2; IGF-1R; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : Affibody molecules are small (7 kDa) affinity proteins of non-immunoglobulin origin that have been generated to specifically interact with a large number of clinically important molecular targets.In this thesis, Affibody molecules have been employed as tracers for radionuclide molecular imaging of HER2- and IGF-1R-expressing tumors, paper I-IV, and for surface knock-down of EGFR, paper V. LÄS MER

  5. 5. Tumor targeted delivery of cytotoxic payloads using affibody molecules and ABD-derived affinity proteins

    Författare :Hao Liu; Torbjörn Gräslund; Stefan Ståhl; Nick Devoogdt; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Targeted tumor therapy; immunotoxins; ADCs; affibody molecule; ADAPT; pseudomonas exotoxin A; maytansinoid; Biotechnology; Bioteknologi;

    Sammanfattning : Cancer treatment cost billions of dollars every year, but the mortality rate is still high. An ideal treatment is the so-called “magic bullets” that recognize and kill tumor cells while leaving normal cells untouched. LÄS MER