Sökning: "Emma Lundberg"

Visar resultat 6 - 10 av 10 avhandlingar innehållade orden Emma Lundberg.

1. 6. Antibody-based subcellular localization of the human proteome

   Författare :Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

   Sammanfattning : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. LÄS MER

3. 7. Towards subcellular localization of the human proteome using bioimaging

   Författare :Charlotte Stadler; Emma Lundberg; Carolina Wählby; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

   Sammanfattning : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. LÄS MER

4. 8. The spatiotemporal protein landscape of human cells

   Författare :Lovisa Stenström; Emma Lundberg; Björn Högberg; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Spatial proteomics; Immunofluorescence; Human Protein Atlas; Nucleolus; Cell cycle; Citizen science; Bioteknologi; Biotechnology;

   Sammanfattning : This thesis focuses on the spatiotemporal mapping of proteins at a subcellular level. In other words, determining the cellular location of proteins over time. From a biological point of view, knowledge about protein location is fundamental to understand protein function. LÄS MER

5. 9. Spatial proteome profiling of the compartments of the human cell using an antibody-based approach

   Författare :Mikaela Wiking; Emma Lundberg; Björn Önfelt; Torbjörn Gräslund; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Fluorescence imaging; Human proteome; Human Protein Atlas; Immunofluorescence; Mitochondria; Organelles; Spatial distribution; Bioteknologi; Biotechnology;

   Sammanfattning : The human cell is complex, with countless processes ongoing in parallel in specialized compartments, the organelles. Cells can be studied in vitro by using immortalized cell lines that represent cells in vivo to a varying degree. LÄS MER

7. 10. On computational methods for spatial mapping of the human proteome

   Författare :Casper F. Winsnes; Emma Lundberg; Ida-Maria Sintorn; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Spatial proteomics; Human Protein Atlas; Micronuclei; Citizen science; Biotechnology; Bioteknologi;

   Sammanfattning : Proteins are complex molecules that are involved in almost every task in the body. In general, the role a protein fulfills is highly dependent on where in the cell it is located, its subcellular localization. LÄS MER