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Visar resultat 1 - 5 av 84 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Chemometrics : Unravelling information from complex data generated by modern instruments

    Författare :Pedro F. M. de Sousa; Magnus Åberg; Johan Trygg; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemometrics; Chromatography; Mass spectrometry; DIA; PCA; PLS; Experimental Design; DNA; adductomics; Analytical Chemistry; analytisk kemi;

    Sammanfattning : Chemometrics is a discipline dedicated to solving problems arising from complicated analytical systems, combining statistics, mathematics, and computational programming languages.This thesis is based on the work developed in four scientific projects published as papers in scientific journals. LÄS MER

  3. 2. Capillary electrophoresis mass spectrometry applied to structural proteomics and small molecule analysis

    Författare :Jordan Aerts; Erik T. Jansson; Per E. Andrén; Petr Man; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary Electrophoresis Mass Spectrometry; Hydrogen Deuterium Exchange Mass Spectrometry; Enantioselective Separations; Drugs of Abuse; Analytical Chemistry; Analytisk kemi; Analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

    Sammanfattning : Capillary electrophoresis with mass spectrometric (CE–MS) detection offers a separation method without equal in terms of flexibility, utility, and cost efficiency. Here we demonstrate precisely this through the application of several laboratory-built CE–MS instruments for the separation of brain metabolites in non-primates, enantioselective separations of synthetic anesthetic metabolites in fractionated pony urine, application in structural proteomics workflows, and identification of exogenous alkaloid biotransformationproducts in human cerebrospinal fluid (CSF). LÄS MER

  4. 3. Mass Spectrometry of Biologically Active Small Molecules : Focusing on polyphenols, alkaloids and amino acids

    Författare :Zdeněk Spáčil; Leopold L. Ilag; Frantisek Turecek; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; LC-MS; LDI; Mass spectrometry; HPLC; UHPLC; polyphenols; phenolic acids; BMAA; Separation methods; Separationsmetoder; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Sammanfattning : The foci of this dissertation are on advanced liquid chromatography (LC) separation and mass spectrometry (MS) techniques for the analysis of small bioactive molecules. In addition to discussing general aspects of such techniques the results from analyses of polyphenols (PPs), alkaloids and amino acids published in five appended studies are presented and discussed. LÄS MER

  5. 4. Development and evaluation of analytical techniques for antibodies and antibody-drug conjugates : From verification of conjugation to stability testing

    Författare :Malin Källsten; Jonas Bergquist; Fredrik Lehmann; Sara Lind; Lucia Kovac; Alain Beck; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody-drug conjugates; drug-to-antibody ratio; monoclonal antibody; mass spectrometry; hydrophobic interaction chromatography; sample preparation; aggregation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Sammanfattning : Antibody-drug conjugates (ADCs) are becoming increasingly important in oncology. ADCs are inherently heterogeneous analytes. For a successful drug candidate to go through to the clinic, extensive characterization and evaluation of the constructs are necessary. LÄS MER

  7. 5. Investigation of Carbohydrate Hydrolysing Enzymes in Applications for Characterisation and Synthesis of Carbohydrates

    Författare :Carina Nilsson; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytisk kemi; enzyme hydrolysis; characterisation of carbohydrates; Analytical chemistry; mass spectrometry;

    Sammanfattning : Cellulose and starch are non-toxic, biodegradable and modifiable polymers. These properties have made them increasingly important in industrial products e. g. in pharmaceutical applications and in technical dispersions. LÄS MER