Sökning: "LC-MS"

Visar resultat 1 - 5 av 176 avhandlingar innehållade ordet LC-MS.

  1. 1. Selectivity in NMR and LC-MS Metabolomics : The Importance of Sample Preparation and Separation, and how to Measure Selectivity in LC-MS Metabolomics

    Författare :Albert Elmsjö; Curt Pettersson; Torbjörn Arvidsson; Thomas Moritz; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; Metabolomics; NMR; LC-MS; HILIC; UHPLC; Q-ToF; selectivity; co-feature ratio; method evaluation; data evaluation; Analytical Pharmaceutical Chemistry; Analytisk farmaceutisk kemi;

    Sammanfattning : Until now, most metabolomics protocols have been optimized towards high sample throughput and high metabolite coverage, parameters considered to be highly important for identifying influenced biological pathways and to generate as many potential biomarkers as possible. From an analytical point of view this can be troubling, as neither sample throughput nor the number of signals relates to actual quality of the detected signals/metabolites. LÄS MER

  2. 2. Analysis of Metabolites in Complex Biological Samples Using LC/MS and Multivariate Data Analysis : Metabolic Fingerprinting and Detection of Biomarkers

    Författare :Helena Idborg; Sven Jacobsson; Per-Olof Edlund; Jonas Bergquist; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Metabolomics; Metabonomics; Metabolic Fingerprinting; Drug Metabolism; Biomarkers; Human Plasma; Rat Urine; LC MS; ZIC-HILIC; UPLC; UHPLC; ToF; Curve Resolution; Peak Detection; Pattern Recognition; Classification; PCA; PLS; PARAFAC; N-PLS; Data Fusion; Hierarchical Modeling; Batch modeling; Data Concatenation; Breast Cancer; Phospholipidosis; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Sammanfattning : To facilitate early diagnosis of diseases and elucidation of the processes involved in their development and progression, various specific compounds or ‘biomarkers’ are often monitored. The first step is to decide which compounds to analyze. LÄS MER

  3. 3. New strategies and tools for optimizing LC-MS data processing in metabolomics

    Författare :Mattias Eliasson; Johan Trygg; Björn Grung; Umeå universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : .... LÄS MER

  4. 4. The Impact of Drugs of Abuse on the Neuroproteome : Application of Immunoblotting and LC-MS-based Proteomics

    Författare :Uwe Rossbach; Pierre Le Grevès; Fred Nyberg; Matthias Hallberg; Jan Kehr; Uppsala universitet; []
    Nyckelord :;

    Sammanfattning : Qualitative and quantitative analysis of proteins can be based either on immunological or on mass spectrometry-utilizing methods. In this thesis, different approaches of protein analysis were applied to elucidate the impact of drugs of abuse on the rat brain. Androgens have been reported to affect mood, anxiety and memory function. LÄS MER

  5. 5. Development of LC-MS/MS Methods for the Analysis of Chiral and Achiral Pharmaceuticals and Metabolites in Aqueous Environmental Matrices

    Författare :Victoria K.H. Barclay; Curt Pettersson; Monika Johansson; Niklas Tyrefors; Mohamed Abdel-Rehim; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; Environmental water matrices; chiral separation; LC-MS MS; trace analysis; method development; active pharmaceutical ingredients; metabolites; Analytical Pharmaceutical Chemistry; Analytisk farmaceutisk kemi;

    Sammanfattning : This thesis describes the development of liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for the trace analysis of active pharmaceutical ingredients (APIs) and their metabolites in aqueous environmental matrices. The research was focused on the development of chiral LC-MS/MS methods for the analysis of fluoxetine and metoprolol, as well as their chiral metabolites in environmental water samples. LÄS MER