Sökning: "carbohydrate-binding modules"
Visar resultat 1 - 5 av 7 avhandlingar innehållade orden carbohydrate-binding modules.
1. Functional studies and engineering of family 1 carbohydrate-binding modules
Sammanfattning : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. LÄS MER
2. Engineering protein-carbohydrate interactions - lessons from natural and evolved carbohydrate binding modules
Sammanfattning : Protein-carbohydrate interactions are of central role in all living organism. In the studies presented in this thesis several strategies for engineering such interactions have been investigated. In the first study the binding affinity of a carbohydrate binding module (CBM) was improved a 100-fold through affinity maturation. LÄS MER
3. Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules
Sammanfattning : Wood and wood products are of great economical and environmental importance, both in Sweden and globally. Biotechnology can be used both for achieving raw material of improved quality and for industrial processes such as biobleaching. LÄS MER
4. Mannan-hydrolysis by hemicellulases: enzyme-polysaccharide interaction of a modular beta-mannanase
Sammanfattning : The enzymatic degradation of plant polysaccharides is a process of fundamental importance in nature which involves a wide range of enzymes. In this work, the structure and function of hemicellulose-degrading enzymes was investigated. The focus was on a beta-mannanase (TrMan5A) produced by the filamentous fungus Trichoderma reesei. LÄS MER
5. On the engineering of proteins: methods and applications for carbohydrate-active enzymes
Sammanfattning : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. LÄS MER