Sökning: "autotransporter"
Visar resultat 1 - 5 av 9 avhandlingar innehållade ordet autotransporter.
1. Surface expression using the AIDA autotransporter : Towards live vaccines and whole-cell biocatalysis
Sammanfattning : The area of surface expression has gathered a lot of interest from research groups all over the world and much work is performed in the area. Autotransporters have been used for surface expression in Gram-negative bacteria. LÄS MER
2. Combinatorial Protein Engineering Of Affibody Molecules Using E. Coli Display And Rational Design Of Affibody-Based Tracers For Medical Imaging
Sammanfattning : Directed evolution is today an established strategy for generation of new affinity proteins. This thesis describes the development of a cell-display method using Escherichia coli for directed evolution of Affibody molecules. LÄS MER
3. Bacterial display systems for engineering of affinity proteins
Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER
4. Impact of glucose uptake rate on recombinant protein production in Escherichia coli
Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER
5. Improved detection and performance of surface expression from the AIDA-I autotransporter
Sammanfattning : Surface expression of recombinant proteins has attracted a lot of attention due to its potential in applications such as enzyme production, vaccine delivery and bioremediation. Autotransporters have been used for surface expression of a variety of proteins, but the expression systems reported in literature have typically been inflexible and incapable of detecting proteolysis, thereby limiting surface expression yield. LÄS MER