Sökning: "staphylococcal display"

Visar resultat 1 - 5 av 26 avhandlingar innehållade orden staphylococcal display.

  1. 1. Bacterial display systems for engineering of affinity proteins

    Författare :Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
    Nyckelord :Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

    Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER

  3. 2. Staphylococcal surface display for protein engineering and characterization

    Författare :John Löfblom; Stefan Ståhl; Henrik Wernérus; K. Dane Wittrup; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; affibody; combinatorial library; epitope mapping; Gram-positive bacteria; protein engineering; staphylococcal surface display; Molecular biology; Molekylärbiologi;

    Sammanfattning : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. LÄS MER

  4. 3. Staphylococcal surface display in directed evolution

    Författare :Nina Kronqvist; Stefan Ståhl; Mats A. A. Persson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; combinatorial library; directed evoluation; Gram-positive bacteria. protein engineering; Bioengineering; Bioteknik;

    Sammanfattning : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. LÄS MER

  5. 4. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

    Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER

  7. 5. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications

    Författare :Mikael Åstrand; Sophia Hober; John Löfblom; Larry Gold; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Protein G; ERBB2; ERBB3; Directed evolution; phage display; staphylococcal display; bispecific; Bioteknologi; Biotechnology;

    Sammanfattning : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. LÄS MER