Sökning: "Cellulose-binding"

Visar resultat 1 - 5 av 14 avhandlingar innehållade ordet Cellulose-binding.

1. 1. Functional studies and engineering of family 1 carbohydrate-binding modules

   Författare :Janne Lehtiö; KTH; []
   Nyckelord :Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

   Sammanfattning : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. LÄS MER

3. 2. Design of enzymatic systems for the modification of cellulose fiber surfaces

   Författare :Malin Gustavsson; KTH; []
   Nyckelord :Candida antarctica lipase B; Pichia pastoris; cellulose-binding module; proteolysis; carbohydrate ester; xyloglucan endotransglycosylase; fiber modification;

   Sammanfattning : This thesis describes the design of enzymatic systems forthe modification of cellulose fiber surfaces. A single-enzymeapproach employed a conjugate between theNeocallimastix patriciarumCel6A cellulose-bindingmodule and lipase B fromCandida antarctica(CBM-CALB) to enhance theaccessibility of the enzyme to the target hydroxyl groups. LÄS MER

4. 3. Strategies for cellulose fiber modification

   Författare :Per Persson; KTH; []
   Nyckelord :Candida antarctica lipase B; cationic catalysis; cellulose-binding module; dendrimer; enzymatic polymerization; fiber modification; silica-cast replica; sol-gel mineralization; organocatalysis; xyloglucan endotransglycosylase;

   Sammanfattning : This thesis describes strategies for and examples ofcellulose fiber modification.The ability of an engineered biocatalyst, acellulose-binding module fused to theCandida antarcticalipase B, to catalyze ring-openingpolymerization of e-caprolactone in close proximity tocellulose fiber surfaces was explored. LÄS MER

5. 4. Expression and Mutagenesis studies of Candida antactica lipase B

   Författare :Johanna C. Rotticci-Mulder; KTH; []
   Nyckelord :Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

   Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER

7. 5. Engineering of staphylococcal surfaces for biotechnological applications

   Författare :Henrik Wernérus; KTH; []
   Nyckelord :affibody; albumin binding protein; Bacterial surface display; cell immobilisation; bioremediation; combinatorial protein engineering; flow cytometry; Gram-positive; metal-binding; staphylococcal protein A; Staphylococcus carnosus; Staphylococcus xylo;

   Sammanfattning : The engineering of bacterial surfaces has in recent yearsattracted a lot of attention with applications in manydifferent areas of bioscience. Here we describe the use of twodifferent surface display systems for the gram-positivebacteria Staphylococcus carnosus and Staphylococcus xylosus invarious biotechnological applications. LÄS MER