Sökning: "single-molecule readout"
Visar resultat 1 - 5 av 13 avhandlingar innehållade orden single-molecule readout.
1. Readout Strategies for Biomolecular Analyses
Sammanfattning : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. LÄS MER
2. Single-Molecule Detection and Optical Scanning in Miniaturized Formats
Sammanfattning : In later years polymer replication techniques have become a frequently employed fabrication method for microfluidic and micro-optical devices. This thesis describes applications and further developments of microstructures replicated in polymer materials. LÄS MER
3. Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization
Sammanfattning : Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte detection, having important implications for monitoring early disease progression and the effects of drug treatment. LÄS MER
4. Proximity Ligation Assays for Disease Biomarkers Analysis
Sammanfattning : One of the pressing needs in the field of disease biomarker discovery is new technologies that could allow high performance protein analysis in different types of clinical material, such as blood and solid tissues. This thesis includes four approaches that address important limitations of current technologies, thus enabling highly sensitive, specific and parallel protein measurements. LÄS MER
5. Detection and Sequencing of Amplified Single Molecules
Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER