Sökning: "Amplified single molecule"
Visar resultat 1 - 5 av 16 avhandlingar innehållade orden Amplified single molecule.
1. Detection and Sequencing of Amplified Single Molecules
Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER
2. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification
Sammanfattning : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. LÄS MER
3. Single-Molecule Detection and Optical Scanning in Miniaturized Formats
Sammanfattning : In later years polymer replication techniques have become a frequently employed fabrication method for microfluidic and micro-optical devices. This thesis describes applications and further developments of microstructures replicated in polymer materials. LÄS MER
4. Development of Electrical Readouts for Amplified Single Molecule Detection
Sammanfattning : Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. LÄS MER
5. Readout Strategies for Biomolecular Analyses
Sammanfattning : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. LÄS MER