Sökning: "Single cell analysis"

Visar resultat 1 - 5 av 647 avhandlingar innehållade orden Single cell analysis.

  1. 1. Live Single Cell Imaging and Analysis Using Microfluidic Devices

    Författare :Mohammad Ali Khorshidi; Helene Andersson-Svahn; Håkan Jönsson; Carolina Wählby; Johan Elf; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

    Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

  3. 2. Technologies for Single Cell Genome Analysis

    Författare :Erik Borgström; Afshin Ahmadian; Sten Linnarsson; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

    Sammanfattning : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. LÄS MER

  4. 3. Imaging-based methods for NK cell studies at the single-cell level

    Författare :Quentin Verron; Björn Önfelt; Niklas Sandström; Michael Dustin; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Immunology; NK cells; microscopy; single-cell; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik;

    Sammanfattning : The immune system provides defense against infectious agents such as viruses, bacteria and parasites. Besides eliminating extracellular agents, the immune system also constantly monitors our own cells for signs of transformation, including tumor development and virus infection. LÄS MER

  5. 4. Droplet microfluidics for single cell and nucleic acid analysis

    Författare :Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

    Sammanfattning : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. LÄS MER

  7. 5. Single Molecule Detection : Microfluidic Automation and Digital Quantification

    Författare :Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER