Sökning: "Bioanalytical assay"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden Bioanalytical assay.

1. 1. Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization

   Författare :Olov Wahlsten; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; QCM-D; biomarker detection; TIRF microscopy.; Bioanalytical assay; spectrofluorometry; fluorescence; single-molecule readout; drug discovery; liposomes;

   Sammanfattning : Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte detection, having important implications for monitoring early disease progression and the effects of drug treatment. LÄS MER

3. 2. Rapid sample preparation and bioanalytical techniques for efficient screening of organic pollutants in the environment

   Författare :Malin Nording; Peter Haglund; Ron Hoogenboom; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; accelerated solvent extraction; CAFLUX; food; immunoassay; PAH; PCDD; PCDF; pressurized liquid extraction; soil; CALUX; cell-based bioassay; chemically activated fluorescent gene expression; chemically activated luciferase gene expression; dioxin; ELISA; enzyme-linked immunosorbent assay; feed; Environmental chemistry; Miljökemi;

   Sammanfattning : Large numbers of samples often need to be prepared and analysed in surveys of organic pollutants in the environment, but while the methods commonly used in such surveys can provide abundant detail they are generally costly, time-consuming and require large amounts of resources, so there is a need for simpler techniques. The work underlying this thesis assessed the potential utility of more convenient sample preparation and bioanalytical techniques for rapidly screening various environmental matrices that could be useful complements to higher resolution methods. LÄS MER

4. 3. New Bioanalytical Approaches, Magneto Immunoassays and SIRE Technology Based Biosensors

   Författare :Kirstin Kriz; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemical technology; Biokemisk teknik; antigens; superparamagnetic nanoparticles; magnetic inductance transducer; competitive magneto immunoassay; enzymes; metabolites; differential measurements; SIRE; biosensor;

   Sammanfattning : This thesis presents two new technologies, SIRE technology based biosensors and magneto immunoassays, which can be used for bioanalytical applications. One of the main problems associated with biosensor technology is the instability of the recognition element since it is of biological origin and thus, has a limited lifetime. LÄS MER

5. 4. Covalent coupling of antibodies to solid phases, in particular to particles and their use in immunoassay

   Författare :Gerd Rundström; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioanalytical engineering; Bioanalytisk teknik;

   Sammanfattning : The immobilisation of antibodies (Ab) or antigens (Ag) to solid phases is always a critical step when developing a heterogeneous immunoassay. There are two main procedures: passive adsorption and covalent coupling. Adsorption is a simple method, but it has its disadvantages including denaturation of antibodies and their leakage from the surface. LÄS MER

7. 5. Development of Electroacoustic Sensors for Biomolecular Interaction Analysis

   Författare :Henrik Anderson; Ilia Katardjiev; Björn Ingemarsson; Inger Vikholm-Lundin; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; biosensor; protein interactions; kinetics; affinity; QCM; quartz crystal microbalance; piezoelectric resonators; dissipation; motional resistance; Bioanalytical engineering; Bioanalytisk teknik; Analytical chemistry; Analytisk kemi; Teknisk fysik med inriktning mot mikrosystemteknik; Engineering Science with specialization in Microsystems Technology;

   Sammanfattning : Biomolecular interaction analysis to determine the kinetics and affinity between interacting partners is important for the fundamental understanding of biology, as well as for the development of new pharmaceutical substances. A quartz crystal microbalance instrument suitable for kinetics and affinity analyses of interaction events was developed. LÄS MER