Sökning: "single-cell"

Visar resultat 41 - 45 av 469 avhandlingar innehållade ordet single-cell.

1. 41. Genetic determinants of epithelial differentiation in the mouse and human lungs

   Författare :Andreas Liontos; Christos Samakovlis; Robert Rottier; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; airway development; single cell; secretory epithelial cells; SCRINSHOT; facultative stem cells; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : Several airway epithelial cell types and subpopulations have been defined, using high throughput single-cell mRNA profiling. However, the spectrum and topology of cell populations within the secretory cell lineage has not been explored. LÄS MER

3. 42. Making Visible the Proximity Between Proteins

   Författare :Carl-Magnus Clausson; Ola Söderberg; Jens Coorssen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proximity ligation assay; In situ PLA; rolling circle amplification; protein interaction; protein-protein interaction; in situ; single cell; single molecule; protein complex; antibody; cancer; tissue section; microscopy; image analysis; system biology; multiplex; dynamic range; methods development; systems biology; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. LÄS MER

4. 43. Computational methods for analysis and visualization of spatially resolved transcriptomes

   Författare :Jose Fernandez Navarro; Joakim Lundeberg; Martin Hemberg; KTH; []
   Nyckelord :RNA; RNA-seq; single cell; scRNA-seq; transcriptomics; spatial transcriptomics; brain; 3D; Alzheimer’s disease; Bioteknologi; Biotechnology;

   Sammanfattning : Characterizing the expression level of genes (transcriptome) in cells and tis- sues is essential for understanding the biological processes of multicellular or- ganisms. RNA sequencing (RNA-seq) has gained traction in the last decade as a powerful tool that provides an accurate quantitative representation of the transcriptome in tissues. LÄS MER

5. 44. Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques

   Författare :Ida Grundberg; Mats Nilsson; Ulf Landegren; Olli-Pekka Kallioniemi; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; padlock probes; in situ; rolling circle amplification; mRNA; genotyping; mutation detection; cancer; tissue sections; diagnostics; single-molecule; single-cell; microscopy; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Sammanfattning : The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. LÄS MER

7. 45. Massively parallel analysis of cells and nucleic acids

   Författare :Julia Sandberg; Joakim Lundeberg; Nader Pourmand; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Massively parallel sequencing; 454; Illumina; multiplex amplification; whole genome amplification; single cell; polyguanine; flow-cytometry;

   Sammanfattning : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. LÄS MER