Sökning: "proximity ligation"

Visar resultat 16 - 20 av 63 avhandlingar innehållade orden proximity ligation.

  1. 16. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

    Författare :Olle Ericsson; Ulf Landegren; Mats Nilsson; Edwin Southern; Uppsala universitet; []
    Nyckelord :Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

    Sammanfattning : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. LÄS MER

  3. 17. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

    Författare :Tonge Ebai; Ulf Landegren; Masood Kamali-Moghaddam; Peter Nilsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

    Sammanfattning : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. LÄS MER

  4. 18. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

    Författare :Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. LÄS MER

  5. 19. Molecular Tools for Detection and Characterization of Proteins and Extracellular Vesicles in Health and Disease

    Författare :Ehsan Manouchehri Doulabi; Masood Kamali-Moghaddam; Lucia Mincheva-Nilsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; High-resolution mass spectrometry; Proximity ligation assay; Proximity extension assay; Thymidine kinase 1; in situ PLA; High-throughput.; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Detecting molecules involved in cancer is critical for cancer research and diagnostics. To achieve this goal, sensitive protein detection is essential to improving the chances of finding, verifying, validating and developing valuable biomarkers. LÄS MER

  7. 20. Biomarkers for Better Understanding of the Pathophysiology and Treatment of Chronic Pain : Investigations of Human Biofluids

    Författare :Anne-Li Lind; Torsten Gordh; Masood Kamali-Moghaddam; Jonas Bergquist; Eva Kosek; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; chronic pain; neuropathic pain; lumbar radicular pain; amyotrophic lateral sclerosis; radiculopathy; fibromyalgia; pathophysiology; spinal cord stimulation; mechanism of action; disc herniation; cerebrospinal fluid; plasma; biomarker; human; protein; chemokines; cytokines; inflammation; neuroinflammation; mass spectrometry; proximity ligation assay; proximity extension assay; antibody suspension bead array; protein profiling; molecular medicine; personalized medicine; Anestesiologi och intensivvård; Anaesthesiology and Intensive Care; Biomedical Laboratory Science; Biomedicinsk laboratorievetenskap; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry; Medicinsk vetenskap; Medical Science; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Chronic pain affects 20 % of the global population, causes suffering, is difficult to treat, and constitutes a large economic burden for society. So far, the characterization of molecular mechanisms of chronic pain-like behaviors in animal models has not translated into effective treatments. LÄS MER