Sökning: "luciferase"
Visar resultat 1 - 5 av 92 avhandlingar innehållade ordet luciferase.
1. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology
Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER
2. Recombinant Enzymes in Pyrosequencing Technology
Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER
3. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors
Sammanfattning : Seven-transmembrane, G-protein coupled receptors play a central role in physiology since they facilitate cell communication in multicellular organisms by recognition of a broad range of ligands. They also represent important drug targets. LÄS MER
4. Evaluating an experimental model consecutive to abdominal wall hernia repair outcome
Sammanfattning : Background: Abdominal wall hernia is a common surgically treated condition. Patients with primary umbilical hernia are operated with suture or mesh repair, but recurrence and complication rates have been debated.Larger abdominal wall hernias need implantation of reinforcing material for repair. Synthetic implants are dominating. LÄS MER
5. Molecular Pharmacology and Structure Function Modelling of the Leukotriene B4 Receptor BLT1
Sammanfattning : Leukotrienes are membrane derived bioactive lipids that play an important role in immune responses by initiating and maintaining the inflammatory responses. Leukotriene B4 (LTB4) released at the site of an inflammatory response attracts, activates and prolongs the life of leukocytes and lymphocytes. LÄS MER